Stage-Specific Differences in Gene Expression of Mouse Kidneys Insulted by Ischemia Reperfusion Injury
- VernacularTitle:小鼠肾脏急性缺血再灌注损伤不同时间点基因表达谱的差异
- Author:
Ning HUANG
1
;
Jing CANG
;
Zhanggang XUE
;
Hao WANG
Author Information
1. 复旦大学附属中山医院麻醉科
- Keywords:
Ischemia reperfusion;
Gene expression;
Mouse kidneys
- From:
Chinese Journal of Clinical Medicine
2015;(3):283-287
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the stage‐specific differences in gene expression of mouse kidneys insulted by ischemia reper‐fusion(IR) injury .Methods:Mouse kidney tissues were collected at 4 h and 24 h after IR .Total RNA of control and IR kidneys were prepared for Microarray and real‐time fluorescence quantitative reverse transcription polymerase chain reaction(RT‐qPCR) analysis .Results:The number of differential expressed genes(DEGs) at 24 h after IR was larger than that at 4 h .The DEGs at 4 h were associated with metabolism and stress responses ,while the DEGs at 24 h were associated with inflammation and apop‐tosis .The numbers of up‐regulated genes at both 4 h and 24 h were larger than those of down‐regulated genes .Several down‐regulated ,up‐regulated ,or unchanged genes were confirmed by RT‐qPCR .Conclusions:Stage‐specific changes of gene expres‐sion occurres during IR injury .These DEGs at early or intermediate‐late stages of IR injury are tightly associated with their stage‐specific pathophysiological processes .
