Tissue Engineering Skin Made of Microskin Combined with Artificial Dermis in the Application of Huge Wound Healing in Rats
- VernacularTitle:微粒皮复合人工真皮构建的组织工程皮肤用于大鼠巨大创面修复的研究
- Author:
Yongzhe TANG
1
;
Qi HE
;
Fazhi QI
Author Information
1. 上海交通大学医学院附属国际和平妇幼保健院乳腺外科
- Keywords:
Microskin;
Tissue engineering skin;
Healing
- From:
Chinese Journal of Clinical Medicine
2015;(2):117-121
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effect of tissue engineering skin(TES) ,which was made of microskin combined with artificial dermis ,in the healing of huge wound on the back of SD rats .Methods:The models of huge wound on the back were made from the selected 32 SD rats at 8 weeks old .They were randomly divided into blank control group (Group A) ,artificial dermis group (Group B) ,microskin group (Group C) and TES group (Group D) .The wounds in Group B ,Group C and Group D were healed by artificial dermis with punching net ,in‐situ microskin autografts ,the TES made of autogenous in‐situ microskin combined with artificial dermis , respectively . The wounds in Group A were covered with vaseline gauze . The situations of wound healing at the 1st ,3rd and 5th week after operation were observed .The effects of ESCs during the wound healing process were observed by the CK15 and CD29 immunohistochemical stalning .The healing quality of dermis on wound was evaluated by HE stalning and Masson stalning of tissue biopsies of the back of rats and observation under scanning electronic microscopy .Results:The best healing effect was in Group D and the wounds were almost healed at the 5th week after grafting .The results of immunohistochemical stalning showed that ESCs of rats in Group D had moved to the epidermal layer gradually and took part in the wound healing .The thickness of dermal layer in Group D was larger than that in the other 3 groups(P<0 .01) and the collagen fibers in dermal layer were arranged compactly and neatly in Group D .Conclusions:The effect of the TES made of microskin combined with artificial dermis in the application of wound healing is superior to that of microskin and artificial dermis .
