Inlfuence of Berberine on Cisplatin Antineoplastic Effect in A549 Cells
10.3779/j.issn.1009-3419.2015.08.03
- VernacularTitle:黄连素在A549细胞中对顺铂抗肿瘤作用的影响及其机制
- Author:
JIANG GUOJUN
1
;
LI LI
;
WU XIAOXIANG
;
DONG SHUYING
;
TONG XUHUI
Author Information
1. 233030 蚌埠，蚌埠医学院药学系药理学教研室，安徽省生化药物工程技术研究中心
- Keywords:
Berberine;
Gap junction;
Connexin 43
- From:
Chinese Journal of Lung Cancer
2015;(8):481-486
- CountryChina
- Language:Chinese
-
Abstract:
Background and objectiveCisplatin is a standard ifrst-line chemotherapeutic agents for treating ad-vanced non-small cell lung cancer. Unfortunately, the clinical application cisplatin is restricted because it induces serious adverse reaction. hTe aim of this study is to investigate the inlfuence and probable mechanism of berberine on cisplatin antineoplastic effect on lung cancer A549 cells.MethodshTe total Cx43 protein amount, localization of Cx43 on cell membrane, and gap junction function were observed atfer the A549 cells were treated with berberine. hTe inlfuence of berberine on the antitumor action of cisplatin was detected by standard colony-forming assay. Protein kinase C (PKC) protein, which regulates the gap junction, was subsequently determined.Results Berberine did not affect cell survival at concentrations of 0 μM to 10 μM in the A549 cells. hTe gap junction function between the cells was enhanced through increased Cx43 protein expression and localization of Cx43 on the membrane atfer berberine treatment. hTe intercellular dye coupling through gap junction increased when the cells exposed to 0.1 μM, 1 μM, 10 μM berberine [33.3% (P=0.002,3), 67.0% (P<0.001), 160.0% (P<0.001)] compared withcontrols. hTis effect was associated with the PKC activity. hTe cispla-tin-induced inhibition of colony growth was enhanced when berberine was combined with cisplatin.ConclusionBerberine can obviously increase the antitumor effect of cisplatin by enhancing the function of the gap junction possibly in A549 cells.
