Exploring the Mechanism of Yishenqinglihuoxue Formula in the Intervention of Chronic Kidney Disease Fibrosis in Rats Based on UHPLC-Q-TOF-MS and Network Pharmacology
10.14148/j.issn.1672-0482.2024.1211
- VernacularTitle:基于UHPLC-Q-TOF-MS和网络药理学探讨益肾清利活血方干预慢性肾病大鼠肾纤维化的作用机制
- Author:
Jinli XIE
1
;
Meng CHENG
;
Yiqing WANG
;
Jing ZHAO
;
Wei SUN
;
Jing TAO
Author Information
1. 南京中医药大学第一临床医学院,江苏 南京 210023
- Keywords:
Yishenqinglihuoxue Formula;
chronic kidney disease;
renal fibrosis;
UHPLC-Q-TOF-MS;
network pharmacology
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2024;40(11):1211-1222
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the potential mechanism of Yishenqinglihuoxue Formula(YSQLF)in treating chronic kidney disease fibrosis in rats based on UHPLC-Q-TOF-MS technology and network pharmacology.METHODS UHPLC-Q-TOF-MS was used for qualitative analysis of Yishenqinglihuoxue Formula-containing serum.Targets of the plasma constituents and the disease were retrieved from SwissTargetPrediction,OMIM,GeneCards,and other databases.Then the protein-protein interaction(PPI)network was constructed and core targets were screened for GO term enrichment and KEGG pathway enrichment.Cytoscape software was em-ployed to construct the"drug-compound-core target-pathway"network and the targets and signaling pathways of Yishenqinglihuoxue Formula against fibrosis were predicted.A model of renal fibrosis was established to verify the core targets and pathway proteins.RE-SULTS A total of 56 constituents migrating to blood of Yishenqinglihuoxue Formula were identified.97 common targets of the constit-uents and the disease and 33 core targets were screened out.KEGG enrichment and PPI network analysis showed that Yishenqingli-huoxue Formula may play a role in the treatment of fibrosis through PI3K/Akt and other pathways.Furthermore,the results of animal experiments showed that Yishenqinglihuoxue Formula could reduce the levels of Scr and BUN,improve fibrosis areas,inhibit the acti-vation of the PI3K/Akt pathway,and reduce the protein expression of TNF-α.CONCLUSION Yishenqinglihuoxue Formula may play a role in the treatment of fibrosis by inhibiting PI3K/Akt signaling pathway and inhibiting TNF-α expression.
