Huangqi Guizhi Wuwu Decoction Ameliorates Focal Cerebral Ischemia-Reperfusion Injury in Rats:Pharmacological and Metabolomies Evi-dences
10.14148/j.issn.1672-0482.2021.0920
- VernacularTitle:黄芪桂枝五物汤改善大鼠局灶性脑缺血再灌注损伤:药理和代谢组学证据
- Author:
Jin-Ying LING
1
;
Qi WANG
;
Hai-Zhen FAN
;
Jing-Ya DAI
;
Xiao-Lan CHENG
Author Information
1. 南京中医药大学附属中西医结合医院,江苏南京210028
- Keywords:
Huangqi Guizhi Wuwu Decoction;
cerebral ischemic-reperfusion;
middle cerebral artery occlusion;
metabolomics;
UP-LC/MS
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2021;37(6):920-929
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the neuroprotective effect of Huangqi Guizhi Wuwu Decoction (HGWD) on cerebral ische-mia injury,and to explore the underlying mechanism.METHODS Fifty rats were randomly divided into 5 groups:sham operation group (sham),middle cerebral artery occlusion (MCAO) model group,and MCAO rats treated with HGWD at 5,10,20 g·kg-1.The first two groups were administered intragastrically (i.g.) with saline for 7 days,while HGWD groups were treated with HGWD at 5,10,and 20 g·kg-1,correspondingly.The neurological deficits scores,cerebral infarct size,lipid peroxidation and inflammatory cytokines levels were used to evaluate the brain ischemic injury.The possible mechanism of HGWD on cerebral ischemia-perfusion inju-ry was investigated by metabolomics.RESULTS HGWD treatment significantly attenuated middle cerebral artery occlusion (MCAO)-induced brain ischemic injury of rats as evidenced by decreasing the neurological deficits scores,cerebral infarct size,as well as lipid per-oxidation and inflammatory cytokines levels.Further metabolomics study indicated that great metabolic disorders in serum were induced following cerebral ischemia/reperfusion injury.Totally 23 metabolites associated with ischemic stroke were proposed as potential bio-markers.The metabolic pathway analysis revealed that MCAO induced brain ischemic injuries mainly by targeting glyoxylate and dicar-boxylate metabolism,and phenylalanine,tyrosine and tryptophan biosynthesis.Correspondingly,HGWD could restore most of the im-balanced endogenous metabolites.CONCLUSION HGWD plays a protective role in focal cerebral ischemia-reperfusion injury in rats,which may be associated with the inhibition of inflammatory effect and improvement of multiple metabolic pathways.
