Pharmacodynamics of Changkang Granules in IBS-D Rats
10.14148/j.issn.1672-0482.2016.0283
- VernacularTitle:肠康颗粒对腹泻型肠易激综合征大鼠的药效学研究
- Author:
Lei SHI
1
;
Ming-Qin SHEN
;
Zhi-Gang WANG
;
Yu-Hui LUO
;
Yin-Yu WANG
;
Xue LI
;
Jian-Ming JU
;
Song-Lin LI
Author Information
1. 南京中医药大学附属中西医结合医院、江苏省中医药研究院中药药理室
- Keywords:
Changkang Granules;
irritable bowel syndrome with diarrhea(IBS-D);
rat
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2016;32(3):283-286
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT:OBJECTIVE To study the therapeutic effect of Changkang Granules in rat with irritable bowel syndrome with Diarrhea(IBS-D) and investigate its mechanism.METHODS The model for IBS-D was established by colorectal distention daily in the neonatal rats between postnatal 8 and 21 days.Sixty SD rats were divided into the normal control group,the model group,positive control group,high-dose,middle-dose,low-dose Changkang Granules group.The treatment lasted for 30 days. The threshold of abdominal withdrawal reflex(AWR) during rectal distension was evaluated.The calcitonin gene-related pep-tide(CGRP) in serum,vasoactive intestinal peptide(VIP)in colon homogenates were evaluated by radioimmunoassay.Immuno-histochemical SABC method to observe expression level of SP of colonic specimens.RESULTS Compared with the normal control group,AWR score increased(P <0.01),The content of serum CGRP,VIP levels in colonic homogenate and expression level of SP in colon decreased markedly in the model group(P <0.01).After administration with Changkang Granules,the AWR scores of the IBS-D rats were reduced(P <0.01).The content of serum CGRP,colon VIP level and expression of SP in colon was increased significantly(P <0.05 ~0.01).CONCLUSION The therapeutic effect of Changkang Granules on IBS-D rat is obvious.The mechanism could be related to inhibition against the excessive decrease of serum CGRP,colon VIP and ex-pression level of SP in colon in IBS-D rats.
