The role of renal tubular epithelial cells in cadmium-induced renal fibrosis
10.3760/cma.j.cn121094-20201116-00632
- VernacularTitle:肾小管上皮细胞在镉诱导的肾脏纤维化中的作用研究
- Author:
Xin CHOU
1
;
Jie LIU
;
Kunpeng MA
;
Yue SHEN
;
Daoyuan SUN
;
Zhen MIN
Author Information
1. 200433 上海，同济大学附属上海市肺科医院中毒科理化实验室
- Keywords:
Cadmium, Cd;
Renal tubular epithelial cell;
Renal fibroblasts;
Renal fibrosis
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2021;39(12):903-909
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of renal tubular epithelial cells in cadmium-induced renal fibrosis.Methods:Established a sub-chronic cadmium exposure mouse model and analyzed the progress of renal fibrosis induced by cadmium exposure through Masson staining and immunohistochemistry, and then a co-culture system of renal tubular epithelial cells and renal fibroblasts was established, the levels of proliferation and activation of renal fibroblasts were detected by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and Western blotting.Results:Sub-chronic cadmium exposure led to weight loss in mice ( P<0.05) , and the levels of β-microglobulin (β-MG) and N-acetyl β-D-glucosaminidase (NAG) in urine were increased in mice exposed to cadmium ( P<0.05) ; pathological analysis revealed that sub-chronic cadmium exposure damaged renal tubular structure, causing infiltration of inflammatory cells and deposition of collagen fiber by Masson ( P<0.05) , which in turn induced kidney fibrosis in mice; the results of in vitro co-culture experiments showed that cadmium-exposed renal tubular epithelial cells accelerated the synthesis of collagen in renal fibroblasts and promoted the proliferation and activation of renal fibroblasts ( P<0.05) . Conclusion:In the progression of cadmium-induced renal fibrosis, a deleterious renal epithelial-fibroblast cross talk was formed, which may be one of the important mechanisms of cadmium-induced renal fibrosis.
