HLA-A Gene Polymorphism Defined by High-Resolution Sequence Based Typing in 161 Northern Chinese Han People
- Author:
Yan CHUNXIA
1
;
Wang RUILIN
;
Li JINGXIANG
;
Deng YAJUN
;
Wu DONGYING
;
Zhang HONGBO
;
Zhang HONGXING
;
Wang LIDONG
;
Zhang CHUNRONG
;
Sun HAIYAN
;
Zhang XIUQING
;
Wang JIAN
;
Yang HUANMING
;
Li SHENGBIN
Author Information
1. The State Laboratory of the Ministry of Health for Forensic Sciences, Xi'an 710061, China;Forensic Department, Medical College of Xi'an Jiaotong University, Xi'an 710061, China;Beijing Genomics Institute,Chinese Academy of Sciences, Beijing 101300, China;
- Keywords:
HLA-A;
polymorphism;
sequence-based typing (SBT);
Han population
- From:
Genomics, Proteomics & Bioinformatics
2003;1(4):304-309
- CountryChina
- Language:Chinese
-
Abstract:
Human leukocyte antigen (HLA) system is the most polymorphic region known in the human genome. In the present study, we analyzed for the first time the HLA-A gene polymorphisms defined by the high-resolution typing methods--sequence-based typing (SBT) in 161 Northern Chinese Han people. A total of 74 different HLA-A gene types and 36 alleles were detected. The most frequent alleles were A*110101 (GF=0.2360), A*24020101 (GF=0.1646), and A*020101 (GF=0.1553); followed by A*3303 (GF=0.1180), A*3001 (GF=0.0590),and A*310102 (GF=0.0404). The frequencies of following alleles, A*0203, A*0205,A*0206, A*0207, A*030101, A*2423, A*2601, A*3201, and A*3301, are all higher than 0.0093. The homozygous alleles include A*020101, A*110101, A*24020101 and A*310102. Heterozygosity (H), polymorphism information content (PIC), discrimination power (DP) and probability of paternity exclusion (PPE) of HLA-A in the samples were calculated and their values were 0.8705, 0.8491, 0.6014, and 0.9475, respectively. These results by SBT analysis of HLA-A polymorphism in Northern Chinese Han population, especially the allele subtypes character, will be of great interest for clinical transplantation, disease-associated study and forensic identification. Implementation of high-resolution typing methods allows a significantly wider spectrum of HLA variation including rare alleles. This spectrum will further be extensively utilized in many fields.
