Effect of Astragalus mongholicus polysaccharides on gene expression profiles of dendritic cells isolated from healthy donors
10.3969/j.issn.1673-4254.2015.12.27
- VernacularTitle:黄芪多糖干预树突状细胞基因表达与抗动脉粥样硬化的机制
- Author:
Chaojun CHEN
1
;
Qiang FU
;
Yuejun LI
;
Zhiliang LI
Author Information
1. 广州市中西医结合医院脑病科
- Keywords:
Astragalus mongholicus polysaccharides;
dendritic cells;
gene expression;
CD36;
IL27;
IFI16
- From:
Journal of Southern Medical University
2015;(12):1802-1805
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the anti-atherosclerosis mechanism of Astragalus mongholicus polysaccharides (APS) by examining its effect on gene expression profiles of the dendritic cells (DCs) from healthy donors. Methods Peripheral blood DCs from healthy donors were incubated with 200 mg/L APS overnight, and changes in the gene expression profiles were investigated using microarray technique and RT-PCR. Result Compared with the control cells, APS-treated DCs showed significantly up-regulated expressions of CD36 (0.97±0.23 vs 5.45±1.14) and IL-27 (1.08±0.22 vs 2.97±0.61) and down-regulated expression of expression of IFI16 (0.98 ± 0.18 vs 0.46 ± 0.11). Conclusion APS can promote the maturation and differentiation of DCs by up-regulating CD36 and IL-27 and down-regulating IFI16, and thus positively affects the occurrence and progression of the atherosclerosis.
