Low expression ofcentrosomal protein 78 (CEP78) is associated withpoor prognosis ofcolorectal cancer patients
10.1186/s40880-016-0121-3
- Author:
MeifangZhang
1
;
TingmeiDuan
;
LiWang
;
JianjunTang
;
RongzhenLuo
;
RuhuaZhang
;
TiebangKang
Author Information
1. State Key Laboratory of Oncology in South China
- Keywords:
Colorectal cancer;
CEP78;
Cell cycle;
Prognosis
- From:Chinese Journal of Cancer
2016;35(10):509-517
- CountryChina
- Language:Chinese
-
Abstract:
Background:Centrosomal protein 78 (CEP78) has been characterized as a component of the centrosome required for the regulation of centrosome-related events during the cell cycle, but its role in human cancers remains unclear. This study aimed to investigate the role and the clinical value of CEP78 in colorectal cancer (CRC).
Methods:Quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry were performed to examine CEP78 expression in CRC tissues and adjacent noncancerous tissues. The association between CEP78
expression and clinical outcomes of CRC patients was determined. The effect of CEP78 on cell growth was examined invitro by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, colony formation, and lfow cytometry assays and invivo using a nude mouse model.
Results:The expression level of CEP78 was signiifcantly lower in tumor tissues than in the adjacent normal tissues (P<0.01). Low CEP78 expression was signiifcantly associated with poor differentiation (P=0.003), large tumor size (P=0.017), lymphatic metastasis (P=0.034), distant metastasis (P=0.029), and advanced stage (P=0.011). Kaplan–Meier analysis indicated that patients with low CEP78 expression had shorter survival than those with high CEP78 expression (P<0.01). Overexpression of CEP78 in CRC cells signiifcantly reduced cell viability and colony formation invitro and halted tumor growth invivo. Further study showed that CEP78 reintroduction in CRC cells resulted in G2/M phase arrest rather than cell apoptosis.
Conclusions:CEP78 might function as a tumor suppressor and serve as a novel prognostic marker in CRC.
