The expression andprognostic value ofprotein tyrosine kinase 6 inearly-stage cervical squamous cell cancer
10.1186/s40880-016-0114-2
- Author:
XiaoJingWang
1
;
YingXiong
;
ZeBiaoMa
;
JianChuanXia
;
YanFangLi
Author Information
1. Sun Yat-Sen University Cancer Center
- Keywords:
Cervical squamous cell cancer;
PTK6;
Prognosis;
Immunohistochemistry
- From:Chinese Journal of Cancer
2016;35(7):374-381
- CountryChina
- Language:Chinese
-
Abstract:
Background:Protein tyrosine kinase 6 (PTK6) is overexpressed in many epithelial tumors and predicts poor progno?sis. However, PTK6 expression status and its role in cervical squamous cell cancer are unknown. This study aimed to investigate the expression level and clinical signiifcance of PTK6 in early?stage cervical squamous cell cancer.
Methods:Quantitative reverse transcription?polymerase chain reaction (qRT?PCR) and western blotting analysis were performed to detect PTK6 mRNA and protein expression levels in 10 freshly frozen, early?stage cervical squamous cell cancer specimens and adjacent non?tumorous cervical tissues. The expression of PTK6 was detected using immuno?histochemical staining in 150 formalin?ifxed, paraffn?embedded, early?stage cervical squamous cell cancer sections and 10 normal cervical tissue sections.
Results:The mRNA and protein levels of PTK6 in cancer tissues were higher than those in adjacent non?tumorous cervical tissues. Immunohistochemical analysis showed that PTK6 was not expressed in normal cervical tissues but was overexpressed in the cytoplasm of cervical squamous cell cancer cells. The level of PTK6 expression was signiif?cantly associated with tumor grade (P=0.020). The 5?year overall survival rate of patients with high PTK6 expression was lower than that of patients with low PTK6 expression (81.3% vs. 96.2%,P=0.008). Multivariate Cox regression analysis showed that the expression level of PTK6 in cervical squamous cell cancer was an independent prognostic factor for patient survival (hazard ratio=5.999, 95% conifdence interval 1.622–22.191,P<0.05).
Conclusions:PTK6 is overexpressed in cervical squamous cell cancer. Increased PTK6 expression is associated with reduced 5?year overall survival. PTK6 expression is an independent prognostic predictor for cervical cancer.
