Effects of E2F-1 overexpression on apoptosis of gastric cancer cells and expressions of apoptosis-related genes
10.3321/j.issn:1000-467X.2009.11.010
- VernacularTitle:E2F-1过表达对胃癌细胞凋亡及相关基因表达的影响
- Author:
Yan LIN-HAI
1
;
Li LEI
;
Xie YUBO
;
Xiao QIANG
;
Wang CHANG-QING
Author Information
1. 广西医科大学附属第一医院
- Keywords:
gastric neoplasm;
E2F-1;
gene expression profile;
differentially expressed genes;
apoptosis
- From:Chinese Journal of Cancer
2009;28(11):1176-1180
- CountryChina
- Language:Chinese
-
Abstract:
Background and Objective: E2F transcription factor 1 (E2F-1) is an important transcription factor in cell cycle. This study was to investigate the effects of E2F-1 overexpression on apoptosis of gastric cancer MGC-803 cells and expressions of the downstream genes. Methods: The apoptotic rates were measured by flow cytometry in MGC-803/E2F-1 cells,MGC-803/EV cells or untransfected MGC-803 cells.The total RNA was extracted from MGC-803/E2F-1 cells or MGC-803 cells,and cDNA was obtained by RTPCR. Fluorescent(fluorescence exchange clip)probes marked by Cy5 and Cy3 were hybridized with gene chips containing 21522 human genes.Subsequently,the two signal images were scanned by Lux Scan 10K/A dual pathways laser scanner and analyzed by LuxScan3.0 image analysis software. RT-PCR was used to verify the target genes. Results: The apoptotic rate of MGC-803/E2F-1 cells[(8.40±0.91)%] was higher than that of MGC-803/EV [(4.53±0.61)%] and MGC-803 cells[(4.97±0.47)%].Fifteen differentially expressed apoptosis-related genes were detected,4 of which were up-expressed and 11 were down-expressed genes,and the same results were verified by RT-PCR. Conclusion: Overexpression of E2F-1 accelerates apoptosis of gastric carcinoma MGC-803 cells,which may be related to the 15 differentially expressed genes.
