Application of virus load testing in HIV antibody screening positive but western blot negative samples
10.3760/cma.j.cn112866-20240412-00061
- VernacularTitle:病毒载量检测在HIV抗体筛查阳性而免疫印迹试验阴性样本中的应用
- Author:
Sheng GE
1
;
Yong TAO
;
Li WANG
Author Information
1. 枣庄市疾病预防控制中心艾滋病防治科，枣庄 277000
- Keywords:
HIV;
Antibody screening;
Viral load;
Western blot
- From:
Chinese Journal of Experimental and Clinical Virology
2024;38(4):464-467
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the application of viral load (VL) testing in human immunodeficiency virus (HIV) antibody screening positive but western blot (WB) negative samples, provide scientific basis for laboratory diagnosis of such populations.Methods:For the HIV antibody retest samples from the initial screening laboratory of Zaozhuang City Center for Disease Control and Prevention from 2021 to 2023, as well as samples from the voluntary counseling and testing at outpatient clinic of our unit that showed a negative WB test result, VL and CD4 + T lymphocyte counts were tested. Four weeks later, the patients were followed up and blood samples were collected by the current district (city) Center for Disease Control and Prevention, and sent to the confirmation laboratory for confirming testing. Results:Among the thirty-two patients, there were fifteen cases with target not detected (TND) by using VL. After four weeks, the follow-up results were all negative. There was one case with a VL test result of 20-5 000 copies/ml, and after four weeks, the follow-up result was positive. There were sixteen cases with a VL test result of >5 000 copies/ml. After four weeks, the follow-up results were all positive. Person correlation analysis was conducted between the log value of VL and CD4 + T lymphocyte count, and the result showed a certain degree of negative correlation ( r=-0.63, P=0.006). Conclusions:VL testing can assist in distinguishing patients with positive HIV antibody screening and negative WB results, and VL testing and WB testing should complement each other, combined with CD4 + T lymphocyte count and epidemiological history to make a diagnosis.
