Correlation of-31G/C Polymorphisms of Survivin Promoter to Tumorigenesis of Gastric Carcinoma
10.3321/j.issn:1000-467X.2008.03.007
- VernacularTitle:Survivin启动子区域-31G/C多态性与胃癌发生的关系
- Author:
Zheng-Jiang CHENG
1
;
Li-Hua HU
;
Shao-Jun HUANG
Author Information
1. 华中科技大学同济医学院附属襄樊医院
- Keywords:
Gastric neoplasm/etiology;
Survivin gene;
Single nucleotide polymorphisms
- From:Chinese Journal of Cancer
2008;27(3):258-263
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND & OBJECTIVE:Survivin,a bifunctional protein that regulates cell division and suppresses apoptosis,may play an important role in tumorigenesis.This study was to determine the correlations of survivin gene 31-GC polymorphisms to the occurrence of gastric carcinoma and survivin mRNA expression.METHODS:The-31G/C single nucleotide polymorphism(SNP)of survivin promoter jn peripheral blood samples from 96 gastric carcinoma patients and 67 healthy subjects was analyzed by polymerase chain reaction-restriction fragment length polymorphism(PCRRFLP)and gene sequencing.Survivin mRNA expression in gastric carcinoma tissues was detected by real-time quantitative reverse transcription polymerase chain reaction (RQ-RT-PCR).RESULTS:The genotype frequencies for-31G/G,-31G/C and-31C/C were 20.84%,39.58% and 39.58% in gastric carcinoma patient,and 46.26%,41.80%and 11.94% in healthy subjects,respectively.The frequencies of survivin-31 C allele and C/C genotype were significantly higher in gastric carcinoma patients than in healthy subjects[59.37%VS.32.84%,X2=22.26,P＜0.005,odds ratio (OR)=2.98,95% confidence interval(CI)=1.96-4.51;39.58%VS.11.94%,x2=14.88,P＜0.005,OR:4.83,95%CI=2.91-8.03],but survivin mRNA was overexpressed with no significant difference in gastric cancer tissues subgrouped by genotypes.CONCLUSION:The-31 C genotype of survivin promoter is associated with gastric cancer,and may be a risk factor of gastric carcinoma.
