Study on Sero-Diagnosis of Nasopharyngeal Carcinoma Using a Dual Antibody Test against Recombinant Epstein-Barr Virus Antigens
10.3321/j.issn:1000-467X.2003.09.002
- VernacularTitle:抗重组EB病毒抗原双重抗体检测血清学诊断鼻咽癌的研究
- Keywords:
Nasopharyngeal carcinoma(NPC);
Sero-diagnosis;
Dualantibody;
Epstein-Barr virus (EBV)
- From:Chinese Journal of Cancer
2003;22(9):903-906
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND & OBJECTIVE: The aim of this study was tooptimize a dual-antibody assay for sero-diagnosis of nasopharyngeal carcinoma(NPC) by evaluating 4 Epstein-Barr virus(EBV) antigen-based enzyme-linkedimmunosorbent assays(ELISAs). METHODS: The serum samples of 57pretreated NPC patients and 58 apparently healthy adults in Guangzhou werecollected. The levels of anti-EBV antibody in the sera were tested by 4 ELISAs,which were developed using fusion proteins of glutathione transferase and EBVspecific recombinant antigens, namely, EBNA1-IgA, EBNA1-IgG, Zta-IgA, andZta-IgG. RESULTS: When evaluated individually, the sensitivity(0.9123) andnegative predictive value(0.9074) of EBNA1-IgA were the highest among the 4ELISAs tested. Zta-IgA test had the highest individual accuracy rate(n, 0.8870)and Youden index(J, 0. 7738) . The dual positives for EBNA1-IgA and Zta-IgAwere the highest among the 4 dual positives when paired ELISAs were evaluated.Five NPC patients with negative reaction to EBNA1-IgA showed positive reactionof Zta-IgA, and 7 NPC patients with negative reaction to Zta-IgA showed positivereaction of EBNA1-IgA. CONCLUSION: The EBNA1-IgA assay is more suitablethan the other 3 ELISAs(EBNA1-IgG, Zta-lgA, and Zta-IgG) when usedindividually for serological diagnosis of NPC. When two assays are combined,EBNA1-IgA and Zta-IgA have complementary effect on serological diagnosis forNPC and is thus an optimal combination of serum antibody assays.
