Expression of LRRC4 Has the Potential to Decrease the Growth Rate and Tumorigenesis of Glioblastoma Cell Line U251
10.3321/j.issn:1000-467X.2003.09.001
- VernacularTitle:LRRC4基因表达能降低胶质母细胞瘤细胞系U251的生长和成瘤潜能
- Keywords:
Glioblastoma;
U251;
LRRC4;
Tumor suppressor
- From:Chinese Journal of Cancer
2003;22(9):897-902
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND & OBJECTIVE: LRRC4 is a novel gene that theauthor has identified recently, which displayed significant downregulation inprimary brain tumor biopsies. This study was designed to investigate if LRRC4has the potential of suppressing brain tumor growth. METHODS: The full-lengthcoding region of LRRC4 gene was subcloned into the expression vectorpcDNA3.1, the recombinant was introduced into the glioblastoma cell line U251by liposome transfection, and the U251 cells stably expressing LRRC4 genewere established by G418 selection. Furthermore, cell proliferation assay, softagar assay, tumorigenesis assay were taken to examine the effect of LRRC4expression on cell growth and tumor formation. RESULTS: U251 cells stablyexpressing full-length coding region of LRRC4 were established bylipofection-mediated transfection and selected for further study. Compared withthe nontransfected and vector-transfected cells, the cells transfected with LRRC4cDNA exhibited a significant increase of expression of LRRC4 mRNA by Northernblot analysis. Further, when cell proliferation was followed over several days, thecells expressing the transfected LRRC4 cDNA grew more slowly thannontransfected cells. Consistently, the cells transfected with LRRC4 exhibitedmarkedly lower colony formation rate. These clones were injected into athymicnude mice who was killed after 40 days and the tumor sizes were evaluated.Tumor volume in mice was significantly smaller in the group of cells stablytransfected with LRRC4 cDNA than in the control. CONCLUSION: LRRC4 genemay be transfected into the human glioblastoma cell line U251. The expression ofLRRC4 in U251 cells may have the potential to suppress tumor cell growth andthe tumorigenesis of U251 cell transplanted in nude mice.
