Short Hairpin RNAs Induced RNA Interference in Human Cells
10.3321/j.issn:1000-467X.2003.06.002
- VernacularTitle:短发夹状RNA在人细胞诱导RNA干扰
- Keywords:
U6 promoter;
RNA;
RNA interference;
Human cell
- From:Chinese Journal of Cancer
2003;22(6):566-570
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND ＆ OBJECTIVE:RNA interference(RNAi) is an evolutionarily conserved posttranscriptional gene silencing, in which the introduction of double- stranded RNA into a cell leads to specific suppression of gene expression. RNAi has become an important tool for gene function studies. The aim of this study was to induce RNAi in mammalian cells by short hairpin RNAs(shRNAs) generated from a DNA vector, therefore, to provide a new approach for gene function analysis. METHODS: Dual Luciferase System was used to assess the intracellular effect of the shRNAs generated from a DNA vector. The inhibitory effect of this DNA vector- driven shRNAs at different condition was measured. RESULTS: shRNAs generated from the DNA vector induced RNAi in human cells, and suppressed gene expression in a sequence- specific manner. The inhibitory effect was highly related to the target sites. CONCLUSION: shRNAs induced RNAi in human cells and suppressed gene expression in a sequence- specific manner. This approach may be used for gene function study.
