Significance of fluorescent histogram of daunorubicin(DNR) in diagnosis of drug resistance to leukemic cells
10.3321/j.issn:1000-467X.1999.03.003
- VernacularTitle:柔红霉素(DNR)荧光直方图诊断白血病细胞耐药的意义
- Keywords:
Daunorubicin(DNR);
Fluorescent histogram;
Drug resistance;
Flow cytometer (FCM);
Leukemia
- From:Chinese Journal of Cancer
1999;18(3):246-249
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop a fluorescent histogram of intracellular daunorubicin(DNR) as a diagnostic method for drug resistance in acute myeloid leukemia(AML).Methods:15 patients with clinically diagnosed drug resistant AML and 5 patients with previously untreated AML were studied. The intensity of fluorescence of intracellular DNR and the fluorescent histogram were determined by flow cytometer(FCM).According to a degree of the intensity, both drug-resistant and-sensitive zones were marked off. Left shift of main peak(LSMP) with the fluoresent histogram may be diagnosed as drug resistance, and right shift of main peak(RSMP) as drug sensitivity. R/S stood for drug resistant index(DRI);R for the number of drug resistant cells, and S for drug-sensitive cells. DRI≥ 2 was drug-resistant or DRI <2 was drug-sensitive. Fluorescent index(FI) was the product of mean fluorescent intensity (MFI) multiplied by DRI. FI≥ 200 meant drug-resistant, while FI< 200 meant drug-sensitive.Results:Among 5 patients with previously untreated AML, 4 patients were diagnosed as drug-sensitive for RSMP, DRI="0.23～" 1.13,FI="50～" 160,and one patient was diagnosed as primary drug-resistant for LSMP,DRI="3.60," FI="367." In 15 patients with clinically drug resistant AML,12 patients were diagnosed as classical resistance for LSMP,DRI="2.59～" 17.72,and FI="265～" 1041,and 3 patients as regrowth drug resistance for RSMP,DRI="0.39～" 0.67,and FI="57～" 83.Conclusion:Clinically, it was a rapid, convenient and visual way, which by FCM mapping the fluorescent histogram of intracellular DNR, in combination with DRI, FI, a clinically drug-resistant manifestation and a cut off point for diagnosis of the fluorescent intensity of drug-resistant cells, we may in good time find and value a type and a degree of drug resistance to AML. Moreover ,it may provide a basis for working out an individually therapeutic scheme and a method for an observation in drug-resistant reversal treatment as well.
