Establishment of detection method for human adenovirus antigen via surface-enhanced Raman scattering immunochromatographic assay
10.3760/cma.j.cn112866-202203-00000
- VernacularTitle:基于SERS免疫层析技术的人腺病毒抗原检测方法建立
- Author:
Liu ZHENZHEN
1
;
Jia XIAOFEI
;
Xiao RUI
Author Information
1. 军事科学院军事医学研究院辐射医学研究所，北京 100850
- Keywords:
Human adenovirus;
SERS;
Au@Ag NPs;
Specificity;
Point of care test
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(3):317-322
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a rapid and ultrasensitive detection of human adenovirus (HAdV) antigen via Au@Ag NPs-based SERS lateral flow immunoassay(LFIA).Methods:Firstly, the Au@Ag NPs modified with dual-layer 5, 5’-dithiobis-(2-nitrobenzoic acid) (DTNB) was synthesized, and conjugated with specific anti-HAdV antibodies to prepare SERS tags. Next, the SERS tags, HAdV and specific detecting antibodies of anti-HAdV on the test lines (T lines) of strips formed the complex structure of sandwich based on the reaction of antigen and antibody, and the rapid detection of HAdV can be achieved by collecting and analyzing the SERS signals on the T lines. Next, the sensitivity, reproductivity and specificity of the method were evaluated, respectively.Results:The proposed SERS-LFIA via dual-layer DTNB-modified Au@Ag NPs can achieve the qualitative and quantitative detection of HAdV within 15 min. The visual result was 10 ng/ml, and limit of detection (LOD) was 0.1 ng/ml with the quantitative range from 0.1 ng/mL to 1 000 ng/ml. Furthermore, this SERS-LFIA displayed great reproducibility and specificity, and there was no cross-reaction with other five emerging infectious virues including dengue virus, yellow fever virus, west nile virus, zika virus and ebola virus.Conclusions:In this study, the proposed SERS-LFIA based on Au/DTNB@Ag/DTNB NPs can accomplish rapid and ultrasensitive detection of HAdV, revealing the great potential in the field of point of care testing.
