A universal primer next-generation sequencing approach to sequence and analyze complete genomes of GⅡ.4 SydneyP31 norovirus
10.3760/cma.j.cn112866-20211013-00183
- VernacularTitle:通用引物高通量测序扩增GⅡ.4 Sydney[P31]型诺如病毒基因组及进化分析
- Author:
Xi ZHU
1
;
Pengfei WANG
;
Yuxiao YAN
;
Qing ZHANG
;
Huiying LI
;
Miao JIN
;
Zhaojun DUAN
Author Information
1. 中国疾病预防控制中心病毒病预防控制所 国家卫生健康委员会医学病毒和病毒病重点实验室，北京 102206
- Keywords:
Norovirus;
Genome;
Phylogenetic analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(1):15-20
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the evolution of predominant Norovirus strain GⅡ.4 Sydney[P31] from 2017 to 2020 in China.Methods:Universal primers and next-generation sequencing technology were applied to establish Norovirus genogroup Ⅱ genome amplification method . Phylogenetic and key sites were analyzed on GⅡ.4 Sydney[P31] strains.Results:Among the 8 GⅡ.4 Sydney[P31] strains, 6 were successfully amplified and the genome sequences were obtained using the preliminarily established GⅡ genome amplification method . Phylogenetic analysis showed that the 6 strains obtained in this study from 2017-2020 strains grouped with the 2015-2019 GⅡ.4 Sydney[P31] reference strains in one cluster, and the Chinese strain GZ20133135 in 2013 and the 2012-2014 GⅡ.4 Sydney[P31] reference strains grouped into another one. A mutation Asp372Asn was found adjacent to the HBGA binding site Ⅱ. Epitope analysis showed that strains after 2017 have developed several aa mutations in A epitopes (297, 372, and 373), B epitopes (333), E epitopes (414), and H epitopes (309, 310), wherein the 2020 strains 20HN261 and 20HN253 have new changes in the A epitope (368) and G epitope (355) compared with the previous strains.Conclusions:The key mutation sites of the Chinese predominant Norovirus strain GⅡ.4 Sydney[P31] have been determined, which provides a scientific basis for tracking the emergence of new strains, and provides basic data for the development of vaccines against epidemic strains in China.
