Comprehensive analysis of microRNA-mRNA co-expression in circadian rhythm.
10.3858/emm.2009.41.9.070
- Author:
Young Ji NA
1
;
Jung Hwan SUNG
;
Suk Chan LEE
;
Young Ju LEE
;
Yeun Joo CHOI
;
Woong Yang PARK
;
Hee Sup SHIN
;
Ju Han KIM
Author Information
1. Seoul National University Biomedical Informatics (SNUBI), Seoul National University College of Medicine, Seoul 110-799, Korea. juhan@snu.ac.kr
- Publication Type:Original Article ; Comparative Study ; Research Support, Non-U.S. Gov't
- Keywords:
circadian rhythm;
gene expression profiling;
gene expression regulation;
liver;
mice;
micro-RNA
- MeSH:
Animals;
*Circadian Rhythm;
Gene Expression Profiling;
Gene Expression Regulation;
Liver/metabolism;
Male;
Mice;
Mice, Inbred C57BL;
MicroRNAs/*genetics;
RNA, Messenger/*genetics
- From:Experimental & Molecular Medicine
2009;41(9):638-647
- CountryRepublic of Korea
- Language:English
-
Abstract:
To investigate the potential role of microRNA (miRNA) in the regulation of circadian rhythm, we performed microarray-based expression profiling study of both miRNA and mRNA in mouse liver for 48 h at 4-hour intervals. Circadian miRNA-mRNA target pair is defined as the pair both elements of which show circadian expression patterns and the sequence-based target relationship of which can be predicted. Circadian initiators, Clock and Bmal1, showed inversely correlated circadian expression patterns against their corresponding miRNAs, miR-181d and miR-191, targeting them. In contrast, circadian suppressors, Per, Cry, CKIe and Rev-erba, exhibited positively correlated circadian expression patterns to their corresponding miRNAs. Genomic location analysis revealed that intronic region showed higher abundance of cyclic than non-cyclic miRNAs targeting circadian genes while other (i.e., 3'-UTR, exon and intergenic) regions showed no difference. It is suggested that miRNAs are involved in the regulation of peripheral circadian rhythm in mouse liver by modulating Clock:Bmal1 complex. Identifying specific miRNAs and their targets that are critically involved in circadian rhythm will provide a better understanding of the regulation of circadian-clock system.
