Prognostic Significance of Amplification of the c-MYC Gene in Surgically Treated Stage IB-IIB Cervical Cancer.
- Author:
Tae Jung KIM
1
;
Ahwon LEE
;
Sung Jong LEE
;
Won Chul LEE
;
Yeong Jin CHOI
;
Kyo Young LEE
;
Chang Suk KANG
Author Information
1. Department of Hospital Pathology, The Catholic University of Korea College of Medicine, Seoul, Korea. klee@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Uterine cervical neoplasms;
In situ hybridization, fluorescence;
MYC;
Hysterectomy;
Prognosis
- MeSH:
Adenocarcinoma;
Carcinoma, Adenosquamous;
Carcinoma, Squamous Cell;
Disease-Free Survival;
Fluorescence;
Genes, myc;
Humans;
Hysterectomy;
In Situ Hybridization;
In Situ Hybridization, Fluorescence;
Multivariate Analysis;
Prognosis;
Recurrence;
Uterine Cervical Neoplasms
- From:Korean Journal of Pathology
2011;45(6):596-603
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Mutations of c-MYC have been described in cervical cancer. However, association between c-MYC gene status and its prognostic significance have not been clarified. METHODS: Tissue microarray sections from 144 patients with stage IB-IIB cervical cancer treated by radical hysterectomy were analyzed by fluorescence in situ hybridization using a region-specific probe for c-MYC and a centromere-specific probe for chromosome 8. RESULTS: Seventy five percent (108/144) of c-MYC gain and 6.9% (10/144) of c-MYC gene amplification were observed. c-MYC gene alteration was more frequently observed in squamous cell carcinoma than adenocarcinoma or adenosquamous carcinoma and were associated with low Ki67 labeling index (p=0.013). c-MYC amplification was not associated with clinicopathologic parameters except absence of bcl2 expression (p=0.048). Survival analysis revealed that patients with c-MYC amplification were significantly associated with higher risk of disease recurrence (p=0.007) and cancer related death (p=0.020). However, c-MYC gain was not associated with unfavorable outcome. Multivariate analysis proved c-MYC amplification as independent prognostic factors of shorter disease free survival and cancer-related death (p=0.028 and p=0.025, respectively). CONCLUSIONS: c-MYC amplification, not gain, is an independent prognostic marker for shorter disease free and cancer specific survival in cervical cancer treated by radical hysterectomy.
