Multiple locus variable number tandem repeat analysis genotype polymorphism of Bacillus anthracis in China
10.3760/cma.j.cn112338-20231212-00350
- VernacularTitle:中国炭疽芽胞杆菌多位点可变数目串联重复序列分析基因型多态性
- Author:
Huijuan ZHANG
1
;
Enmin ZHANG
;
Jinrong HE
;
Wei LI
;
Jianchun WEI
Author Information
1. 传染病溯源预警与智能决策全国重点实验室，中国疾病预防控制中心传染病预防控制所，北京 102206
- Keywords:
Bacillus anthracis;
Genotyping;
Multiple locus variable number tandem repeat analysis;
Genetic polymorphism
- From:
Chinese Journal of Epidemiology
2024;45(7):990-996
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the multiple locus variable number tandem repeat analysis (MLVA) genotype polymorphism of Bacillus (B.) anthracis and establish a MLVA genotype database of B. anthracis in China. Methods:B. anthracis strains isolated from different sources in China since 1947 were collected. Genotype identification was carried out using the MLVA15 genotyping protocol based on 15 variable number tandem repeat loci. The genotypes were uniformly numbered and named. The distribution characteristics of the MLVA genotypes of strains were analyzed. Software Bionumerics was used to construct clustering diagrams to analyze the genetic relationships. Results:The MLVA15 clustering analysis subdivided the isolates into 4 major groups and 91 genotypes, 54 of which were unique to China. The genotypes from MLVA15-CHN1 to MLVA15-CHN6 were widely distributed throughout China and in all eras, while other genotypes were restricted to certain regions or eras.Conclusions:This study established a MLVA genotype database of B. anthracis, which provides basis for the understanding of MLVA genetic polymorphisms and the control and molecular source tracing of the anthrax outbreaks in China.
