Studies on the manufacture and immunogenicity of purified rabies vaccines on hum ans Vero cell

VernacularTitle:精制Vero细胞狂犬病疫苗的研制及免疫学效果观察
From: Chinese Journal of Epidemiology 2001;22(1):17-19
CountryChina
Language:Chinese
Abstract: Objective　Using Vero cell as basic cultur al material to improve the quality of rabies vaccines and to produce rabies va ccines for humans. Methods　CTN-1V10 strain were used for prodaction. Vero cell of 150th generation were used for cultivation. Rotating cultivatal method with rotating bottle was used. Fluids with virus were collected at diffe rent time. Puritied rabies vaccine was produeed on Vero cell after clarification , condensation, purification and extermination. A batch of vaccines made b y this techniques were used for immunological observation. Sixty-three people were injected with this rabies vaccine according to the procedure of time of exposure. Thirty of them were injected with vaccines made in France (Verorab ) while the others were injected with vaccines to be tested. Side effect and neu tratizing antibody were recorded. Results　The quality of this newly developed rabies vaccines has met the quality set by WHO. After all dosages of injection, the rates of positive antibody were both 100% in two groups. The neutralizing antibody among testing group was 11.9 4 IU/ml comparing with control as 11.69 IU/ml. Conclusion　Purified rabies vaccines on Vero cell for humans had reasonable manufac ture technique and less little effect with good technological imnunogenicity.