Use of serum anti-desmoglein-1 anti-desmoglein-3 antibodies in diagnosis of pemphigus vulgaris
10.3760/cma.j.issn.1002-0098.2011.s1.011
- VernacularTitle:两种检测方法在寻常型天疱疮诊断中的作用
- Author:
Hong-Mei ZHANG
1
;
Qin LIN
;
Hong HUA
Author Information
1. 北京大学口腔医学院(口腔医院)
- Keywords:
Pemphigus;
Desmogleins;
Enzyme-linked immunosorbent assay;
Autoantibodies
- From:
Chinese Journal of Stomatology
2011;46(z1):45-49
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of the enzyme-linked immunosorbent assay(ELISA) and indirect immunofluorescent(IIF) on the diagnosis and monitoring of the activity of pemphigus vulgaris (PV).Methods Serum antibody titers of 44 PV patients and 29 none-PV patients were detected by desmoglein(Dsg)-1,3-ELISA and monkey oesphagus-IIF (MO-IIF).The sensitivity and specificity of the ELISA and MO-IIF were compared in the diagnosis of pemphigus vulgaris.The correlation between serum level of anti-desmoglein antibodies and the disease severity was analyzed.Results The sensitivity of MO-IIF was 93％ (41/44) and the specificity was 83 ％ (24/29).The sensitivity of Dsg-3-ELISA was 89％ (39/44) and the specificity was 93％ (27/29).There was no significant difference between MO-IIF and Dsg-3-ELISA (P ＞ 0.05 ).Dsg-3 antibody levels showed positive correlation with oral disease severity (P ＜ 0.01 )and no correlation with skin disease severity in pemphigus vulgaris( P ＜0.05 ).Dsg-1 antibody levels showed positive correlation with skin disease severity ( P ＜ 0.01 ) and no correlation with oral disease severity in pemphigus vulgaris(P ＜ 0.05 ).Conclusions Dsg-3-ELISA and MO- Ⅱ F methods were highly sensitive and specific in the diagnosis of PV.Dsg-1,3-ELISA is the useful tool for the sero-diagnosis and monitoring of the activity of PV.
