Expression of transforming growth factor-β1 mRNA during mouse molar morphogenesis

VernacularTitle:转化生长因子β1 mRNA在鼠磨牙形态发生过程中表达的研究
From: Chinese Journal of Stomatology 2001;36(2):130-132
CountryChina
Language:Chinese
Abstract: Objective　To investigate the temporal and spatial expression and localization of transforming growth factor-β1 mRNA during mouse molar morphogenesis. Methods　The distribution pattern of TGF-β1 mRNA was analysed on 5 μm serial sections of paraformaldehyde-fixed and paraffin-embedded embryonic mouse heads or mandibles using in situ hybridization. Results　The results show that TGF-β1 mRNA was expressed during mouse molar morphogenesis in a temporally and spatially regulated fashion. Local expression of TGF-β1 mRNA in the dental epithelium at bud-staged (E13) and cap-staged (E15) teeth were observed. During bell stage (E16-18), TGF-β1 mRNA was very abundant in the ameloblast layer and dental papilla cells. The expression of TGF-β1 was increased in the layer of odontoblast and ameloblast with the differentiation of these cells. Conclusions　These results suggest that TGF-β1 may have an important role in tooth morphogenesis, and TGF-β1 acts as a paracrine and autoinducing factor.