Effect of calcium hydroxide on human pulp cell intracellular calcium ion in vitro
10.3760/j.issn:1002-0098.2001.02.015
- VernacularTitle:氢氧化钙对人牙髓细胞内游离钙的影响
- From:
Chinese Journal of Stomatology
2001;36(2):128-129
- CountryChina
- Language:Chinese
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Abstract:
Objective To investigate the influence of calcium hydroxide to intracellular calcium ion of human pulp cells in vitro. Methods The sixth generation of human pulp cells were maintained in RPMI 1640 medium supplemented with Fluo-3 probe and Ca(OH)2 for restimulation. The intracellular calcium ion concentration was detected by laser confocal microscope. Results The intracellular calcium ion level in Ca(OH)2 pretreatment group increased after Ca(OH)2 stimulation,while it didn′t response to Ca(OH)2 stimulus in group without Ca(OH)2、CaCl2 or NaHCO4 pretreatment. Conclusion It is important to pretreat the pulp cells with Ca(OH)2 in order to increase the intracellular calcium.
