Determination of lacidipine in Beagle dog plasma by UHPLC-MS/MS method
10.13699/j.cnki.1001-6821.2024.20.026
- VernacularTitle:用UHPLC-MS/MS法测定比格犬血浆中拉西地平的浓度
- Author:
Yan-Yan WANG
1
;
Yi-Hong JIANG
;
Xiao-Ying ZHAO
;
Zhen-Yu ZHOU
;
Xiao-Hong LI
;
Cheng-Da YAN
;
Feng QIN
Author Information
1. 沈阳药科大学药学院,辽宁本溪 117000
- Keywords:
lacidipine;
ultra-high performance liquid chromatography-tandem mass spectrometry;
drug plasma concentration
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(20):3038-3041
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an ultra high performance liquid chromatography-tandem mass spectrometry method for the determination of lacidipine in plasma of beagle dogs was established.Methods It was pretreated by protein precipitation method and the internal standard was nimodipine.Chromatographic column:ACQUITYUPLC? BEH C8(2.1 mm x50.0 mm,1.7 μm),mobile phase:100％water containing 5 mmol·L-1 ammonium acetate-100％acetonitrile,flow rate:0.7 mL·min-1,column temperature:40 ℃,automatic injector temperature:4 ℃,injection volume:20 μL.Electrospray ionization source,positive ion mode,multi-reaction monitoring.The specificity,residual effect,standard curve and quantitative lower limit,precision and recovery,matrix effect and stability of the method were investigated.Results Lacidipine has a good linear relationship in the range of 0.10-50.0 ng·mL-1,r=0.996 6,the lower limit of quantification was 0.10 ng·mL-1.The specificity was good.The intra-and inter-relative standard deviation was less than 12％.The extraction recovery was higher than 80％,and the stability was good.Conclusion The method has the advantages of high sensitivity,simple operation and short analysis time,and was suitable for the pharmacokinetic study of lacidipine in Beagle dog plasma.
