Effects of forsythinol on apoptosis of hepatocellular carcinoma cells through the JAK2-STAT3 signaling pathway
10.13699/j.cnki.1001-6821.2024.19.016
- VernacularTitle:连翘环己醇通过JAK2-STAT3信号通路对肝癌细胞凋亡的影响
- Author:
Xin ZHANG
1
;
Dong-Xiang HUANG
;
Can-Hui TANG
;
Zhi-Piao HUANG
Author Information
1. 江西中医药高等专科学校药学系,江西抚州 344100
- Keywords:
forsythinol;
liver cancer;
Janus kinase 2/signal transduction and transcriptional activator 3;
matrix metalloproteinase 2;
matrix metalloproteinase 9
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(19):2837-2841
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of forsythinol(Fo)on the expression of matrix metalloproteinase-2(MMP2)in hepatoma cells through Janus kinase 2/signal transduction and transcriptional activator 3(JAK2/STAT3)signaling pathway.Methods SMMC-7721 cells were divided into experimental-L,-M,-H groups,control group,inhibitor group and activator group.The control group was added with equal volume dimethyl sulfoxide(DMSO);the experimental-L,-M,-H groups were treated with 50,200,500 μg·mL-1 Fo;and the inhibitor group was added with 50 μmol·L-1 JAK2/STAT3 inhibitor AG490 based on the experimental-M group.In the activator group,10 μmol·L-1 JAK2/STAT3 activator Broussonin E was added to the experimental-M group.Apoptosis was detected by deoxynucleotide terminal transferase-mediated dUTP notch end labeling(TUNEL);protein expression was detected by Western blot;real-time quantitative polymerase chain reaction(qRT-PCR)was used to detect mRNA levels.Results The apoptosis rates of control group,experimental-M group,inhibitor group and activator group were(19.94±4.88)％,(27.04±5.27)％,(15.36±3.40)％and(46.66±7.89)％,respectively;the relative expression levels of phosphorylated JAK2 protein were 1.00±0.13,0.73±0.11,1.33±0.17 and 0.26±0.07,respectively;the relative expression levels of phosphorylated STAT3 protein were 1.00±0.12,0.27±0.04,0.88±0.13 and 0.12±0.04,respectively;the mRNA relative expression levels of MMP2 were 1.00±0.14,0.68±0.08,1.17±0.17 and 0.51±0.09,respectively.Compared with experimental-M group and control group,inhibitor group and activator group,there were statistically significant differences(P＜0.05,P＜0.001).Conclusion Fo promotes apoptosis of hepatocellular carcinoma cells,and its mechanism may be related to the effect of Fo on the expression of MMP2 by regulating JAK2-STAT3 signaling pathway.
