Effects of emodin on inflammatory response in preeclampsia rats by regulating AMPK/TXNIP/NLRP3 signaling pathway
10.13699/j.cnki.1001-6821.2024.14.014
- VernacularTitle:大黄素调节AMPK/TXNIP/NLRP3信号通路对子痫前期大鼠炎症反应的影响
- Author:
Fen CHEN
1
;
Fei-Fei YUAN
;
Wei LI
;
Hong-Li XI
;
Hong-Yan GUO
Author Information
1. 湖北医药学院附属襄阳市第一人民医院妇产科,湖北襄阳 441000
- Keywords:
emodin;
AMP activated protein kinase;
thioredoxin-interacting protein;
NOD-like receptor pyrin domain-containing protein 3;
preeclampsia;
inflammatory response
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(14):2068-2072
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of emodin on inflammatory response in preeclampsia(PE)rats by regulating the AMP activated protein kinase(AMPK)/thioredoxin-interacting protein(TXNIP)/NOD-like receptor pyrin domain-containing protein 3(NLRP3)signaling pathway.Methods PE rat model was established by subcutaneous injection of L-arginine methyl ester(100 mg·kg-1).Sixty female rats were randomly divided into control group,model group,emodin group(40 mg·kg-1 emodin),Compound B10 group(100 mg·kg-1 Compound B10),emodin+Compound B10 group(40 mg·kg-1 emodin+100 mg·kg-1 Compound B10),with 12 rats in each group.The control group and the model group were intraperitoneally injected with the same amount of 0.9％NaCl.The 24 h urine was collected,and the total urinary protein content was determined by Coomassie brilliant blue method.The protein levels of AMPK/TXNIP/NLRP3 signaling pathway were detected by Western blot.Results The total urinary protein levels of control group,model group,emodin group,Compound B10 group and emodin+Compound B10 group were(54.34±6.26),(136.37±15.43),(76.38±8.61),(215.39±25.14)and(110.93±13.92)g·L-1,respectively;urine volume were(10.59±0.92),(15.38±1.49),(11.51±1.13),(21.49±2.50)and(14.71±1.49)mL,respectively;AMPK protein levels were 0.63±0.06,1.57±0.18,0.81±0.09,2.34±0.23 and 1.38±0.15,respectively;TXNIP protein levels were 0.33±0.04,0.79±0.08,0.49±0.10,1.13±0.12 and 0.82±0.09,respectively;NLRP3 protein levels were 0.46±0.05,0.83±0.09,0.56±0.07,1.25±0.14 and 0.78±0.08,respectively.The above indexes:Model group was compared with control group,emodin group and Compound B10 group,emodin+Compound B10 group was compared with emodin group,the differences were statistically significant(all P＜0.05).Conclusion Emodin may alleviate inflammatory reaction in PE rats by inhibiting AMPK/TXNIP/NLRP3 signal axis,thereby improving placental injury.
