Resarch of resveratrol improving interstitial lung disease in rats through TGF-β1/Smad3 signaling transduction pathway
10.13699/j.cnki.1001-6821.2024.12.019
- VernacularTitle:白藜芦醇通过TGF-β1/Smad3信号转导途径改善间质性肺病模型大鼠的研究
- Author:
Xiao-Jing QI
1
;
Na ZHANG
;
Yue-Qin ZHANG
Author Information
1. 青海省人民医院 呼吸重症科,青海 西宁 810007
- Keywords:
resveratrol;
interstitial lung disease;
rat;
bleomycin;
transforming growth factor-β1
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(12):1789-1793
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of resveratrol(RSV)in interstitial lung disease(ILD).Methods The ILD model rats were established by administering a single intratracheal instillation of bleomycin.The ILD model rats were randomly divided into model group and experimental-L,-M,-H groups,with 12 rats each group,then chose another 12 normal rats as blank group.The experimental-L,-M,-H groups received 25,50 and 100 mg·kg-1 RSV by gavage;blank and model groups were given an equal volume of 0.9％NaCl by gavage.Five groups were treated for 14 days with once a day.The levels of pro-inflammatory factors,superoxide dismutase(SOD)in lung tissue were detected by enzyme linked immunosorbent assay.The expression levels of EMT-related proteins and transforming growth factor-β1(TGF-β1),SMAD family member 3(Smad3)were detected by Western blot.Results The levels of interleukin-1 β in the experimental-M,-H groups,model group and blank group were(7.32±0.33),(5.07±0.39),(11.28±0.72)and(3.78±0.12)pg·mL-1;the levels of SOD were(4.73±0.06),(6.55±0.15),(1.21±0.12)and(8.27±0.56)pg·mL-1;the relative expression levels of TGF-β1 protein were 0.49±0.06,0.34±0.04,0.78±0.08 and 0.11±0.02;the relative expression levels of Smad3 protein were 0.44±0.07,0.19±0.03,0.93±0.08 and 0.07±0.01,respectively.The differences of above indexes were statistically significant between the model group and the experimental-M,-H groups(all P＜0.05).Conclusion RSV can protect against ILD rats by inhibiting EMT and downregulating the TGF-β1/Smad3 signaling pathway.
