Effects of protopanaxadiol on the proliferation and apoptosis of human ovarian cancer cells HO-8910 through regulation
10.13699/j.cnki.1001-6821.2024.12.015
- VernacularTitle:原人参二醇对人卵巢癌细胞HO-8910增殖和凋亡的影响
- Author:
Rui-Hua NIE
1
;
Wei ZHU
;
Jin-Yan LUO
Author Information
1. 江西中医药大学岐黄国医书院,江西南昌 330025
- Keywords:
protopanaxadiol;
ovarian cancer;
protein kinase B;
B cell lymphoma-2;
proliferation;
apoptosis
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(12):1769-1773
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of propanaxanediol on proliferation and apoptosis of human ovarian cancer cell HO-8910 and analyze the mechanism.Methods Human ovarian cancer cell HO-8910 were randomly divided into blank group(0.9％NaCl)and experimental-L,-M,-H groups(25,50,100 mg·kg-1 propanaxanadiol treated cells,respectively).Cell counting kit-8(CCK-8)was used to detect the proliferation inhibition;the apoptosis was detected by flow cytometry;the migration was detected by cell scratch test;the invasion was detected by Transwell assay;the expression levels of phosphatidylinositol 3 kinase(PI3K),phosphorylated protein kinase B(p-AKT),proliferating cell nuclear antigen(PCNA),B cell lymphoma-2(Bcl-2)and Bcl-2 associated X protein(Bax)were detected by Western blot.Results The proliferation inhibition rates of blank group and experimental-L,-M,-H groups were(16.89±4.15)％,(28.43±3.66)％,(37.96±4.98)％and(50.11±5.24)％;the apoptosis rates were(4.23±1.07)％,(12.36±2.79)％,(24.32±2.93)％and(42.40±3.28)％;the expression levels of PCNA protein were 0.85±0.08,0.69±0.06,0.43±0.06 and 0.25±0.03;the expression levels of Bax protein were 0.18±0.03,0.33±0.04,0.50±0.05 and 0.69±0.05;the expression levels of Bcl-2 protein were 0.82±0.07,0.63±0.04,0.47±0.05 and 0.30±0.03;the mobility were(52.33±4.25)％,(40.16±4.03)％,(29.63±3.25)％and(20.15±2.12)％;the invasion rates were(60.26±5.88)％,(49.33±4.28)％,(30.15±3.68)％and(22.15±1.96)％;the expression levels of PI3K protein were 0.48±0.04,0.34±0.04,0.26±0.03 and 0.15±0.01;the expression levels of p-AKT protein were 0.45±0.03,0.35±0.02,0.23±0.03 and 0.13±0.02,respectively.There were statistically significant differences in the above indexes between the experimental-L,-M,-H groups and the blank group(all P＜0.05).Conclusion Protopanaxadiol may inhibit the proliferation,migration and invasion of human ovarian cancer cell HO-8910 by blocking the activation of PI3K/AKT signaling pathway,regulating the expression of PCNA,Bax and Bcl-2 proteins and promoting their apoptosis,thus achieving anti-ovarian cancer effects.
