Effects of Ginkgo Biloba extract regulating PI3K/AKT signaling pathway on proliferation and apoptosis of human cerebral vascular adventitia fibroblasts
10.13699/j.cnki.1001-6821.2024.11.003
- VernacularTitle:银杏叶提取物调控PI3K/AKT信号通路对人脑血管外膜成纤维细胞增殖和凋亡的影响
- Author:
Xiao-Chen BI
1
;
Xiao-Bin KONG
;
Na ZHAO
;
Jing-Jing GU
Author Information
1. 浙江中医药大学基础医学院实验教学中心,浙江杭州 310000
- Keywords:
Ginkgo biloba extract;
phosphoinositol 3 kinase/protein kinase B signaling pathway;
human cerebral adventitia fibroblasts;
proliferation;
apoptosis
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(11):1556-1559
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of Ginkgo biloba extract(GBE)on the proliferation and apoptosis of human cerebral adventitia fibroblasts(HBVAFs)by regulating the phosphatidylinositol 3 kinase(PI3K)/protein kinase B(AKT)signaling pathway.Methods HBVAFs cells were divided into blank group(normal culture,no treatment),experimental-L,-M,-H groups(25,50 and 100 mg·L-1 GBE treatment for 24 h)and combined group(100 mg·L-1 GBE treatment and 2 nmol·L-1 insulin-like growth factor-1 treatment for 24 h).Cell proliferation was detected by thiazole blue assay,cell apoptosis was detected by flow cytometry,and the protein expressions of phosphorylated-PI3K(p-PI3K),PI3K、phosphorylated-AKT(p-AKT)and AKT were detected by Western blot.Results The cell survival rates of experimental-L,experimental-M,experimental-H,combined and blank groups were(66.78±8.21)％,(53.51±7.40)％,(42.33±6.01)％,(75.69±5.87)％and(96.77±12.80)％;the apoptosis rates were(24.83±2.47)％,(30.07±3.06)％,(37.02±4.66)％,(16.71±2.11)％and(8.99±0.19)％;p-PI3K/PI3K ratios were 0.75±0.07,0.62±0.06,0.43±0.04,0.72±0.05 and 0.90±0.09;p-AKT/AKT ratios were 0.70±0.07,0.60±0.06,0.38±0.03,0.69±0.06 and 0.90±0.09,respectively.The above indexes of the experimental-L,-M,-H groups were compared with the blank group,and the above indexes of the combined group were compared with the experimental-H group,the differences were statistically significant(all P＜0.05).Conclusion GBE can reduce the proliferation of HBVAFs,promote apoptosis,and its mechanism is related to the inhibition of PI3K/AKT signaling pathway.
