Isotope dilution HPLC-MS/MS method for simultaneous determination of four antimicrobials in children with small volume of plasma
10.13699/j.cnki.1001-6821.2024.10.024
- VernacularTitle:用同位素稀释-HPLC-MS/MS法同时检测儿童微量血浆中4种抗菌药物的浓度
- Author:
Zhi-Mei YANG
1
;
Ya-Bin QIN
;
Yu HAN
;
Xi-Juan JIANG
Author Information
1. 河北医科大学第四医院急诊科,河北石家庄 050011;河北医科大学研究生学院,河北石家庄 050017
- Keywords:
meropenem;
linezolid;
voriconazole;
posaconazole;
high performance liquid chromatography-tandem mass spectrometry;
therapeutic drug monitoring;
children
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(10):1507-1511
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method for simultaneous determination of meropenem,linezolid,voriconazole and posaconazole in children with small volume of plasma,and to use the method in children therapeutic drug monitoring(TDM).Methods The plasma samples were precipitated protein by methanol.And then,the analytes were gradient eluted on an EVO-C18 column by HPLC-MS/MS with mobile phase consisted of water(0.1％formic acid)-acetonitrile(0.1％formic acid)at the flow rate of 0.5 mL·min 1.Ions were monitored in the multiple reaction monitoring(MRM)mode,using positive ion electrospray ionization(ESI).Results Meropenem had a good liner relationship in 0.5-64.0 μg·mL-1(r=0.999 2),0.2-25.6 μg·mL-1 for linezolid and voriconazole(r=0.999 2,r=0.999 9),and 0.1-12.8 μg·mL-1 for posaconazole(r=0.998 9).Accuracy,precision,matrix effect and stability studies all met the requirements.This method was fully verified,and applied to determine the plasma antimicrobial concentrations of 49 pediatric patients.Conclusion The method is simple,rapid,sensitive and suitable for children's plasma concentration monitoring of four antimicrobials.
