Effects of Angelica Sinensis and Astragalus capsules on myocardial autophagy in rats with heart failure
10.13699/j.cnki.1001-6821.2024.10.013
- VernacularTitle:当归黄芪胶囊对心力衰竭大鼠心肌自噬的影响
- Author:
Xue WU
1
;
Xin-Fang LÜ
;
Xiao-Dong ZHI
;
Xin-Ke ZHAO
;
Ying-Dong LI
Author Information
1. 甘肃中医药大学中西医结合学院,甘肃兰州 730030;兰州大学第二医院心血管内科,甘肃兰州 730030;甘肃省中医药防治慢性疾病重点实验室,甘肃兰州 730030;兰州大学第二临床医学院,甘肃兰州 730030
- Keywords:
Angelica sinensis and Astragalus capsule;
heart failure;
autophagy;
phosphatidylinositol 3 kinase;
protein kinase B;
mammalian target of sirolimus;
microtubule associated light chain protein 3-Ⅱ/Ⅰ
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(10):1453-1457
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether Angelica Sinensis and Astragalus capsules(AAC)regulates myocardial autophagy in heart failure rats via the phosphatidylinositol 3 kinase(PI3K)/protein kinase(Akt)/mammalian target of sirolimus(mTOR)signaling pathway.Methods A rat model of heart failure was constructed by intraperitoneal 2.5 mg·kg-1 doxorubicin,and another 8 rats served as the control group.The modeling rats were randomly divided into model group,control group and experimental-L,-M,-H groups.Control group was given 30 mg·kg-1 3-methyladenine by intraperitoneal injection;experimental-L,-M,-H groups were given 150,300 and 450 mg·kg-1 AAC by gavage,respectively;blank and model groups were given the same quantity of sterile distilled water.Six groups were administered once daily for 6 weeks.The cardiac function was measured by ultrasound,and the expression levels of PI3K,Akt,mTOR,sequestosome 1(P62)and microtubule-associated light chain protein 3-Ⅱ/Ⅰ(LC3 Ⅱ/Ⅰ)in myocardial tissue were measured by Western blot.Results In the blank,model,control and experimental-H groups,the left ventricular ejection fraction values were(85.00±3.63)％,(56.75±4.83)％,(75.63±3.70)％and(72.75±4.23)％;the relative expression levels of PI3K were 1.00±0,0.28±0.05,0.64±0.08 and 0.74±0.16;phosphorylated Akt/Akt were 1.00±0,0.49±0.06,0.90±0.16 and 0.95±0.10;phosphorylated mTOR/mTOR values were 1.00±0,0.42±0.09,0.73±0.13 and 0.83±0.08;the relative expression levels of P62 proteins were 1.00±0,0.24±0.12,0.57±0.09 and 0.96±0.10;the relative expression levels of LC3 Ⅱ/Ⅰ proteins were 1.00±0,4.31±0.75,2.20±0.76 and 1.59±0.24,respectively.Compared to the model group,statistical significant were identified in the experimental-H and control groups(all P＜0.05).Conclusion AAC can regulate PI3K/Akt/mTOR pathway,inhibit myocardial autophagy and improve cardiac function in rats with heart failure.
