Determination of lorlatinib concentration in human plasma by two-dimensional high performance liquid chromatography
10.13699/j.cnki.1001-6821.2024.09.018
- VernacularTitle:用二维高效液相色谱法测定人血浆中洛拉替尼的浓度
- Author:
Wei LI
1
;
Jing ZHAI
;
Ming-Hui LONG
;
Yong PAN
;
De-Hua LIAO
Author Information
1. 湖南省肿瘤医院、中南大学湘雅医学院附属肿瘤医院药学部,湖南长沙 410003
- Keywords:
lorlatinib;
two-dimensional high performance liquid chromatography;
therapeutic drug monitoring
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(9):1327-1330
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of lorlatinib in human plasma by two-dimensional high performance liquid chromatography.Methods In the two-dimensional high performance liquid chromatography method,one-dimensional column SX1E-1A(50 mm × 3.5 mm,5 μm)and two-dimensional column SCB-C18(125 mm × 4.6 mm,5 μm)were used with flow rates of 0.8 mL·min-1 and 1.0 mL·min-1,respectively.The column temperature was 40 ℃,The UV detection wavelength was 317 nm,and the sample size was 500 μL.This study investigated the specificity,standard curve and minimum quantification limit,precision and recovery rate,as well as stability of the method.Results The concentration of lolatinib in human plasma showed a good linear relationship in the range of 11.72-1 018.98 ng·mL-1,and the regression equation was y=944.50x-588.90(R2=0.999 7).The minimum limit of quantification was 11.72 ng·mL-1.The extraction recovery rates of the three quality control samples were 97.61％-99.86％,and the intra-day and inter-day precisions were less than 5.29％,indicating that the detection performance of the method was good.Conclusion The method has the characteristics of good stability,high sensitivity and strong anti-interference ability,and is suitable for the determination of loratinib in human plasma.
