Clinical application and determination of nine sedative-hypnotics in human plasma by HPLC-MS/MS
10.13699/j.cnki.1001-6821.2024.08.025
- VernacularTitle:用HPLC-MS/MS法同时测定人血浆中9种镇静催眠类药物浓度及其临床应用
- Author:
Chun-Ling MA
1
;
Bin-Bin CHEN
;
Xiang-Zhen XU
;
Shun TU
;
Hai-Yan LU
Author Information
1. 厦门市仙岳医院、厦门医学院附属仙岳医院、福建省精神医学中心、福建省精神疾病临床医学研究中心临床药学科,福建厦门 361012
- Keywords:
sedative-hypnotics;
high performance liquid chromatography tandem mass spectrometry;
therapeutic drug monitoring
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(8):1208-1212
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish and validate a method for simultaneous determination of 9 sedative-hypnotics in human plasma,and to explore the preliminary clinical application.Methods Plasma samples were precipitated with acetonitrile and determined by high performance liquid chromatography tandem mass spectrometry.The column was Agilent Poroshell 120 EC-C18(2.1 mm × 50.0 mm,2.7μm)and eluted with acetonitrile water containing 0.1％formic acid in an equal degree program at a flow rate of 0.3 mL·min-1.The column temperature was 20 ℃ and injection volume was 5 μL.The deprotonated ions of analytes were ionized by positive ion,electron spray ionization and multiple reaction monitoring mode.The specificity,standard curve and lower limit of quantification,precision and recovery,matrix effect,stability and dilution effect of the method were investigated.Results Excellent linear relationship with correlation coefficient of r2 ≥ 0.997 7 was obtained.The linear of esazolam,alprazolam,oxazepam,clonazepam,lorazepam,triazolam,midazolam,diazepam and zolpidem were 18-1 800,4.5-450,25-2 500,3.5-350,25-2 500,1.5-150,5.5-550,35-3 500,4-400 ng·mL-1,respectively.The lower limit of quantification were 18,4.5,25,3.5,25,1.5,5.5,35,4 ng·mL-1.The method was accurate and precise with acceptable intra-day and inter-day precisions(relative standard deviations were less than 20％for a lower limit of quantification and less than 15％for other quality control samples)and an accuracy of 86.21％-112.38％.The extraction recovery rate were 93.07％-110.50％.The matrix factors normalized by internal standard were 86.61％-108.41％,relative standard deviations were less than 15％.Plasma samples remained stable under various storage conditions.The precision and accuracy of plasma samples were acceptable after dilution.Conclusion The method is simple,rapid,sensitive and specific,and it can be used for simultaneous detection of the 9 sedative-hypnotics in human plasma.
