Effect and mechanism of Jichuan decoction on intestinal function in rats model of slow transit constipation
10.13699/j.cnki.1001-6821.2024.08.021
- VernacularTitle:济川煎对慢传输型便秘大鼠肠道功能的影响及作用机制的研究
- Author:
Gui-Bing PEI
1
;
Bo ZHANG
;
Zhi-Cheng CHEN
;
Hua JIANG
Author Information
1. 东南大学附属中大医院肛肠外科,江苏南京 210009
- Keywords:
Jichuan decoction;
slow transit constipation;
vasoactive intestinal peptide;
substances P;
intestinal flora
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(8):1189-1193
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Jichuan decoction on defecation in rats with slow transit constipation(STC).Methods A total of 36 SD rats were divided into model group,experimental group and control group.Except the control group,STC rat models were established in the other two groups by gavage of 2％rhein acid suspension.The experimental group was given 170％Jichuan decoction of 10 mL·kg-1 by gavage,while the control group and model group were given with 0.9％NaCl by gavage,once a day for 30 days.Fecal dry-wet weight ratio and colon transport time were observed by fecal weighing and recording of first fecal discharge time;substance P(SP)and vasoactive peptide(VIP)contents in plasma were detected by kit;changes in intestinal flora were detected by real-time fluorescent quantitative polymerase chain reaction(qRT-PCR).Results The dry-wet ratio of feces in control group,model group and experimental group were(44.86±4.40)％,(53.44±3.52)％and(44.28±4.33)％,respectively;the colon transport time were(340.17±34.91),(424.67±37.26)and(377.36±38.87)min,respectively;plasma SP were(79.86±7.56),(60.30±2.41)and(78.84±5.83)pg·mL-1,respectively;plasma VIP were(30.56±2.87),(23.38±6.62)and(28.39±2.37)pg·mL-1,respectively;the numbers of Bifidobacterium were 7.77±0.04,5.19±0.09 and 7.45±0.03,respectively;the numbers of Lactobacillus were 6.40±0.07,4.10±0.05 and 6.36±0.08,respectively.There were statistically significant differences in the above indexes between model group and control group,and between experimental group and model group(P＜0.01,P＜0.05).Conclusion Jichuan decoction can improve the defecation of rats with slow transit constipation.
