Effect of Shexiang Baoxin pill on cardiac angiogenesis in spontaneously hypertensive rats
10.13699/j.cnki.1001-6821.2024.08.019
- VernacularTitle:麝香保心丸对自发性高血压大鼠心脏血管新生的影响
- Author:
Rong HUA
1
;
Qing-Hai ZHANG
;
Yi TANG
;
Qian LI
;
Yao-Yuan XIAO
;
Lin-Lin LIU
;
Ming-Xiang TANG
Author Information
1. 湖南省人民医院、湖南师范大学附属第一医院心血管内科,湖南长沙 410000
- Keywords:
Shexiang Baoxin pill;
spontaneously hypertensive rats;
angiogenesis;
vascular endothelial growth factor
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(8):1180-1183
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of Shexiang Baoxin pill on cardiac tissue angiogenesis in spontaneously hypertensive rats(SHR).Method Twenty 12 week old male SHR were randomly divided into experimental group and model group,with 12 week old male SD rats as the normal control group.The experimental group rats were orally administered with Shexiang Baoxin pill(45 mg·kg-1)daily,and their blood pressure was monitored using a non-invasive tail artery blood pressure gauge every four weeks.Eight weeks later,cardiac tissue was taken for platelet endothelial cell adhesion molecule-1(PECAM-1/CD31)immunofluorescence staining to observe CD31 expression level.Use protein blotting to detect the expression levels of myocardial endothelial growth factor(VEGF),myocardial endothelial growth factor receptor 2(VEGF-R2),basic fibroblast growth factor(bFGF)and phosphorylated protein kinase B(p-Akt)/protein kinase B(Akt)proteins.Result There was significant increase in blood pressure between the experimental group,model group and normal group at the same time point(all P＜0.01),but there was no statistically significant difference in blood pressure changes between the experimental group and model group at the same time point(all P＞0.05).The CD31 expression rates of the normal group,model group and experimental group were(3.79±0.84)％,(2.54±0.42)％and(3.56±0.49)％;VEGF levels were 0.95±0.10,0.73±0.08 and 0.94±0.15;VEGF-R2 levels were 0.85±0.10,0.61±0.14 and 0.80±0.10;bFGF levels were 0.84±0.04,0.51±0.21 and 0.74±0.14;p-Akt/Akt levels were 0.85±0.15,0.57±0.13 and 0.80±0.20,respectively.The differences between the normal group and the model group,as well as the experimental group and the model group,were statistically significant(all P＜0.05).Conclusion Shexiang Baoxin pill can promote the neovascularization of microvessels in the heart tissue of spontaneously hypertensive rats,and its mechanism may be related to the activation of PI3K/Akt phosphorylation,upregulation of bFGF,VEGF and their receptor VEGF-R2 in myocardial tissue.
