Effects of mannose on inhibiting proliferation and inducing apoptosis of HeLa cells in cervical cancer
10.13699/j.cnki.1001-6821.2024.06.011
- VernacularTitle:甘露糖抑制宫颈癌HeLa细胞增殖和诱导凋亡的影响
- Author:
Li-Cui YE
1
;
Jing-Yi WANG
;
Rui XIANG
;
Dan NIE
Author Information
1. 西南医科大学临床医学院,四川泸州 646000;成都医学院第二附属医院·核工业四一六医院妇产科,四川成都 610000
- Keywords:
mannose;
cervical cancer;
proliferation;
apoptosis;
Wnt/β-catenin pathway
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(6):834-838
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of mannose on proliferation and apoptosis of HeLa cells of cervical cancer and its effect on Wnt/β-catenin pathway.Methods Cervical cancer HeLa cells were cultured in vitro and divided into control group(without any treatment)and low,medium and high dose experimental groups(cells treated with 5,10 and 25 mmol·L-1 mannose).Cell counting kit 8(CCK-8)and flow cytometry were used to detect cell proliferation and apoptosis.The B cell lymphoma-2(Bcl-2),p21,Bcl-2 associated X protein(Bax)and Wnt/β-catenin pathway-related proteins were detected by Western blot.Results The cell inhibition rates of control group and low,medium,high dose experimental groups were(0.00±0.13)％,(11.25±3.42)％,(23.78±3.41)％and(35.98±4.52)％;apoptosis rates were(4.59±0.35)％,(11.45±1.32)％,(18.47±2.01)％and(25.69±2.43)％;p21 protein expression were 0.21±0.03,0.34±0.04,0.51±0.06 and 0.69±0.05;the Bcl-2 protein expression were 0.89±0.06,0.67±0.04,0.52±0.05 and 0.35±0.06;Bax protein expression were 0.34±0.05,0.49±0.06,0.65±0.07 and 0.81±0.06;Wnt1 protein expression were 0.74±0.07,0.60±0.05,0.46±0.05 and 0.32±0.04;β-catenin protein expression were 0.80±0.06,0.65±0.05,0.47±0.06 and 0.29±0.05.The above indexes showed statistically significant differences between control group and low,medium and high dose experimental groups(all P＜0.05).Conclusion Mannose inhibits proliferation and induces apoptosis of HeLa cells of cervical cancer,and the mechanism may be related to the inhibition of Wnt/β-catenin pathway.
