Effects of Anchusa italica Retz.on cough variant asthma and the level of TLR4/NF-κB signaling pathway
10.13699/j.cnki.1001-6821.2024.05.014
- VernacularTitle:意大利牛舌草对咳嗽变异性哮喘大鼠TLR4/NF-κB信号通路的影响
- Author:
Liang CHEN
1
;
Xiao-Qin XU
;
Wen-Hui GUO
;
HAJIAKBER·Aisa
;
Di-Na MAI
Author Information
1. 中国科学院新疆理化技术研究所省部共建新疆特有药用资源利用国家重点实验室培育基地,新疆维吾尔自治区乌鲁木齐 830011;中国科学院新疆理化技术研究所中国科学院干旱区植物资源化学重点实验室,新疆维吾尔自治区乌鲁木齐 830011;中国科学院大学新疆理化技术研究所,北京 100049;新疆医科大学附属中医医院新疆中药炮制研究重点实验室,新疆维吾尔自治区乌鲁木齐 830000;新疆维吾尔自治区中医药研究院,新疆维吾尔自治区乌鲁木齐 830000;新疆医科大学第四临床医学院,新疆维吾尔自治区乌鲁木齐 830017
- Keywords:
Anchusa Italica Retz.;
cough variant asthma;
Toll-like receptor;
nuclear factor-kappa B
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(5):698-702
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Anchusa italica Retz.extract on cough variant asthma(CVA)and its mechanism of action.Methods Forty-eight SD rats of each eight were randomly divided into blank group(equal amount of saline),model group(equal amount of saline),control group(250 mg·kg-1 prednisone acetate)and experimental-A1,-A5,-A7 groups(61.1 mg·kg-1 Anchusa italica Retz.extract A1,48.8 mg·kg-1 Anchusa italica Retz.extract A5,201.8 mg·kg-1 Anchusa italica Retz.extract A7),continuous gavage for 28 d;except for the blank group,the other groups were made with ovalbumin-complete Freund's adjuvant.The number of cough times of each rat was observed.The changes in the expression of Toll-like receptor 4(TLR4),myeloid differentiation factor(MyD88)and nuclear factor κB(NF-κB)proteins were observed in the lung tissue.Results The cough times in the blank group,model group,control group and experimental-A1,-A5,-A7 groups were 1.10±0.22,8.33±1.24,3.08±0.65,3.31±0.99,3.08±1.02 and 3.06±0.68;the relative expression levels of TLR4 protein were 0.61±0.01,0.84±0.04,0.66±0.02,0.64±0.04,0.64±0.03 and 0.69±0.02;the relative expression levels of MyD88 protein were 0.54±0.08,0.86±0.06,0.71±0.06,0.65±0.05,0.64±0.08 and 0.70±0.06;the relative expression levels of p-NF-κB protein were 0.48±0.11,0.94±0.06,0.80±0.08,0.68±0.04,0.68±0.06 and 0.78±0.09.Compared with the experimental-A1,-A5,-A7 groups,control group,normal group,the differences were statistically significant in the model group(all P＜0.05).Conclusion The extract of Anchusa Italica Retz.can inhibit the symptoms of CVA rats,and the mechanism may be related to the inhibition of TLR4/MyD88/NF-κB signaling pathway.
