Effect of genistein on lung inflammation in acute exacerbation of chronic obstructive pulmonary disease model rats
10.13699/j.cnki.1001-6821.2023.24.019
- VernacularTitle:金雀异黄酮对慢性阻塞性肺疾病急性加重期模型大鼠肺组织炎症反应的影响
- Author:
Xiao-Xia ZHANG
1
;
You-Tao WU
Author Information
1. 济宁医学院医学检验实验室,山东济宁 272067
- Keywords:
Genistein;
acute exacerbation of chronic obstructive pulmonary disease;
inflammatory response;
Mucus secretion;
toll-likereceptor4/nuclear factor kappa B pathway
- From:
The Chinese Journal of Clinical Pharmacology
2023;39(24):3633-3637
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the inhibitory effect and mechanism of genistein intervention on inflammation in acute exacerbation of chronic obstructive pulmonary disease(AECOPD)rats.Methods The AECOPD model was established by smoking combined with airway infusion of endotoxin and nasal inoculation.The rats were randomly divided into control group,model group,dexamethasone group and low,high dose experimental groups,with 10 rats in each group.Rats in low,high dose experimental groups were intragaically given 7 mg·kg-1 and 14 mg·kg-1 genistein,once a day,until the end of modeling.In the dexamethasone group,1 mg·kg-1 dexamethasone solution was given intragastatically once a day for 5 days at the end of modeling.Rats in control group and model group were given the same amount of distilled water by intragastric administration.The lung function of each group was detected at 24 h after completion;the total number of inflammatory cells and the number of lymphocytes,macrophages and neutrophils in alveolar lavage fluid were detected by Reichs-Giemsa staining;serum neutrophil elastase(NE)and inflammatory factors were detected by the kit;real-time fluorescence quantitative polymerase chain reaction(RT-qPCR)was used to detected the levels of Mucin 5 AC(MUC5AC)mRNA;the expression levels of related proteins were detected by Western blot.Results The levels of forced expiratory volume in 0.3 second/forced vital capacity(FEV0.3/FVC)in control group,model group,low dose experimental group,high dose experimental group and dexamethasone group were(81.57±8.58)％,(53.34±2.75)％,(64.42±6.09)％,(76.24±5.30)％and(79.53±4.73)％;the lymphocyte numbers were(80.19±6.88)× 106,(169.52±57.28)× 106,(130.17±9.01)× 106,(103.55±8.43)× 106 and(84.79±7.31)× 106·L-1;NE levels were(2.02±0.36),(3.44±0.16),(3.15±0.24),(2.34±0.15)and(2.36±0.13)ng·mL-1;TNF-α levels were(9.43±0.62),(17.08±1.69),(14.04±1.47),(10.18±0.98)and(9.70±0.98)ng·mL-1.Compared with control group,the above indexes in model group were statistically significant(all P＜0.05);the above indexes of low dose experimental group,high dose experimental group and dexamethasone group were statistically significant compared with model group(all P＜0.05);the above indexes of high dose experimental group were significantly different compared with low dose experimental group(all P＜0.05).Conclusion The genistein can inhibit the expression of inflammatory factors,NE,MUC5AC proteins,and improve the inflammatory response and mucus secretion.
