Protective effect of Ginkgo biloba extract on oxidative stress injury of brain neurons after subarachnoid hemorrhage in rats
10.13699/j.cnki.1001-6821.2023.24.017
- VernacularTitle:银杏提取物对大鼠蛛网膜下出血后脑神经元氧化应激损伤的保护作用
- Author:
Yi-Ming GUAN
1
;
Jia-Hui TAN
Author Information
1. 黑龙江省中医医院、黑龙江省中医药科学院针灸脑病科,黑龙江哈尔滨 150036
- Keywords:
Ginkgo biloba extract;
subarachnoid hemorrhage;
brain neurons;
oxidative stress
- From:
The Chinese Journal of Clinical Pharmacology
2023;39(24):3623-3627
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of Ginkgo biloba extract on the oxidative stress injury of brain neurons after subarachnoid hemorrhage and the effect of hematoma absorption in rats.Methods Forty male SD rats were randomly divided into sham operation group,model group and low,high dose experimental groups.Ten rats in sham operation group underwent craniotomy and drilling,while the other 30 rats were established rat model of subarachnoid hemorrhage,each group 10 rats.Both the sham operation group and model group were given intraperitoneal injection of 0.9％NaCl in equal amounts,low and high dose experimental groups were given 20 and 100 mg·kg-1·d-1 Ginkgo biloba extract via intraperitoneal injection continuously for 14 d.The neurological deficit scores and the level of cerebral hemorrhage for each rat group were compared.Brain tissue damage was observed by hematoxylin and eosin(HE)staining.Cell apoptosis rate was evaluated by terminal-deoxynucleoitidyl transferase mediated nick end labeling(TUNEL)method.Furthermore,the presence of interleukin(IL)-1 and IL-6,IL-1 β,tumor necrosis factor-α(TNF-α),superoxide dismutase(SOD)and malondialdehyde(MDA)levels were detected by enzyme-linked immunosorbent assay.Results HE pathological staining revealed that the experimental group exhibited notable protection against brain tissue damage,manifested by orderly arranged brain tissue cells,substantially reduced nuclear apoptosis,and disappearance of edema.The neurological deficit scores after treatment in sham operation group,model group and low,high dose experimental groups were 0,2.82±0.32,2.13±0.53 and 1.33±0.26;the bleeding volume were 0,(8.03±1.18),(5.73±1.06)and(3.47±0.94)mL;the apoptosis rates of nerve cells were(3.74±0.83)％,(57.70±8.13)％,(26.83±3.14)％and(10.17±1.89)％,respectively,all with statistically significant differences(all P＜0.05).Levels of IL-1,IL-6,IL-1 β and TNF-α in the brain tissue of low and high dose experimental groups of rats were significantly lower than those in model group(all P＜0.05).Moreover,the SOD levels in the brain tissue of the low and high dose experimental groups were significantly higher than those in model group,whereas the MDA levels were significantly lower(all P＜0.05).Conclusion Ginkgo biloba extract can help protect brain tissues and reduce oxidative stress and damage to brain tissues by subarachnoid space.
