Expression and role of angiogenesis regulatory protein-2 in angiogenesis and transfection induced by high glucose in human umbilical vein endothelial cells
10.13699/j.cnki.1001-6821.2019.07.023
- VernacularTitle:血管生成调节蛋白-2在高糖诱导人脐静脉内皮细胞血管生成与转染中的表达和作用
- Author:
Qing HAN
1
;
Xiao-Yun HE
;
Su-Xian ZHOU
;
Yan-Hua XIAO
Author Information
1. 桂林医学院 附属医院 内分泌科
- Keywords:
blood sugar;
angiogenic regulatory protein-2;
human umbilical vein endothelial cell;
angiogenesis
- From:
The Chinese Journal of Clinical Pharmacology
2019;35(7):678-681
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and effect of angiogenic regulatory protein-2 (VASH2) in high glucose-induced angiogenesis of human umbilical vein endothelial cells (HUVECs) . Methods HUVECs were divided into three groups: normal control group, high glucose group and mannitol group before transfection. The VASH2 negative control plasmid and si-VASH2 were transfected into high glucose-inducing HUVECs, respectively; named si-NC group and si-VASH2 group, and untreated cells were named blank group. The expression of VASH2 and angiogenesis-related factors in each group were detected by real time fluorescence quantitative polymerase chain reaction. Proliferation of HUVECs was detected by MTT assay. Results Before transfection, the expressions of VASH2 mRNA in HUVECs of normal control group, mannitol group, and high glucose group were 1. 02 ± 0. 34, 1. 10 ± 0. 37 and 1. 67 ± 0. 49, respectively; the differences between high glucose group with normal control group and mannitol group were statisticallysignificant (P < 0. 05) . After transfection, the expressions of VASH2 in blank group, si-NC group and si-VASH2 group were 1. 71 ± 0. 57, 1. 68 ± 0. 55 and 1. 01 ± 0. 34, respectively; the expressions of VEGFR2 in the three groups were 1. 77 ± 0. 58, 1. 76 ± 0. 57 and 1. 05 ± 0. 35, respectively; the proliferation rates of HUVECs after transfection for48 h in the three groups were (20. 34 ± 6. 74) ％, (20. 65 ± 6. 75) ％ and (5. 53 ± 1. 51) ％, respectively; the differences of the three factors between si-VASH2 group with blank group and si-NC group were statistically significant (all P < 0. 05) . Conclusion VASH2 is highly expressed in HUVECs induced by high glucose, and interfering with the expression can inhibit the expressions of angiogenesis-related factors, thus participating in the angiogenesis process.
