Protective effect of multi -glycoside of Tripterygium wilfordii Hook.F. on salt-sensitive hypertension-induced renal injury in mice
10.13699/j.cnki.1001-6821.2018.19.017
- VernacularTitle:雷公藤多苷对盐敏感性高血压小鼠肾损害的保护作用
- Author:
Su-Xiao LIU
1
;
Chun-Dong SONG
;
Lin CUI
;
Yuan GAO
;
Shi-Yang XIE
;
Si SHEN
;
Ming-Jun ZHU
;
You-Ping WANG
Author Information
1. 河南中医药大学 第一附属医院 中心实验室及心脏中心
- Keywords:
multi -glycoside of Tripterygium wilfordii Hook.F.;
salt -sensitive hypertension;
renal injury;
monocyte/macrophage;
inflammation
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(19):2302-2305
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clarify the protective effect of multi -glyco-side of Tripterygium wilfordii Hook.F.( GTW) on salt-sensitive hyper-tension-induced renal injury in mice .Methods To establish salt -sensitive hypertension model ,the mice were subjected to uninephrectomy and deoxycorticosterone acetate (DOCA)-salt treatment.The mice were assigned into control group , model group and test group.The control group treated by uninephrectomy was gavaged with 0.9%NaCl;the model group treated by DOCA -salt was gavaged with 0.9%NaCl; the test group treated by DOCA-salt was gavaged with GTW 23 mg· kg-1· d-1. All of the three groups were continuously gavaged twice a day for 5 weeks.The excretion of urinary albumin and 8-isoprostane,creatinine clearance ,the changes in renal pathology and monocyte/macrophage infil-tration were compared among the three groups .Results After treat-ment, the excretion of urinary albumin for the test group , control group and model group were (22.49 ±3.97),(5.70 ±0.98) and (48.75 ±4.49) μg· 24 h-1;the excretion of 8-isopros-tane for the three groups were (1.84 ±0.30 ),(0.42 ±0.08 ) and (3.07 ±0.47 ) ng· 24 h -1;the creatinine clea-rance for the three groups were (245.02 ±27.86 ),(336.53 ±25.99 ) and(133.76 ±18.18 ) mL· 24 h -1;the glome-rulosclerosis index for the three groups were (0.27 ±0.06),(0.09 ±0.04)and (0.64 ±0.10);the tubulointerstitial injury score for the three groups were (1.25 ±0.29),(0.34 ±0.16)and (3.10 ±0.46);the renal F4/80 positive cells for the three groups were(18.84 ±5.36),(9.80 ±1.72) and (63.00 ±9.13)cell· mm-2.Compared with the test group and the control group ,the differences in all of the parameters described above in the model group were statis-tically significant(all P<0.05).Conclusion GTW could ameliorate renal injury in DOCA -salt hypertension via in-hibiting monocyte/macrophage infiltration.
