Determination of temozolomide concentration in human plasma by liquid chromatographytandem mass spectrometry
10.13699/j.cnki.1001-6821.2018.09.029
- VernacularTitle:液质联用测定血浆替莫唑胺的浓度
- Author:
Gang CHEN
1
;
Jin WANG
;
Ze-Juan WANG
;
Xiao-Yi KONG
;
Xiao-Na LIU
;
Chen LIU
;
Ying LIU
;
Lu QI
;
Bao-Li ZHOU
;
Wen-Bin LI
;
Xing-He WANG
Author Information
1. 首都医科大学附属北京世纪坛医院药物Ⅰ期研究室
- Keywords:
liquid chromatographytandem mass spectrometry;
plasma concentration;
temozolomide
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(9):1111-1114
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a rapid,sensitive and specific analytical method based on liquid chromatographytandem mass spectrometry (LC-MS/MS) for the determination of temozolomide concentration in human plasma in equivalence trial clinical trial.Methods After protein sediment by methanol and acetonitrile (1 ∶ 2,0.1％ formic acid),the analyte and internal standard were separated on a Kinetex Ci8 column (3.0 mm × 50 mm) using a isocratic elution consisting of 0.1％ formic acid water solution 0.1％ formic acid and methanol (9∶1) as the mobile phase at a flow rate of 0.3 mL · min-1 The detection was performed using an API 5500 QTrap mass spectrometer in the positive electrospray ionization (ESI) mode.The multiple reaction monitoring (MRM) transitions were m/z 195.1→m/z 138.0 for the analyte and m/z 198.1→m/z 138.0 for temozolomide-d3.Results The calibration curve exhibited a linear dynamic range of 0.05-30 μg · mL-1 (r ＞ 0.997 3).The intra-and inter-day precisions (relative standard deviation,RSD) were 1.06％-7.13％ and 1.80％-5.19％.The intraand interday precisions were 91.02％-109.33％ and 95.14％-101.22％.The recoveries and matrix effects were satisfactory in all the biological matrices examined.Conclusion This method was successfully used for the determination of temozolomide concentration in human plasma in equivalence trial.
