Effects of celecoxib on the expression of Survivin and neuron apoptosis after traumatic brain injury in rats
10.13699/j.cnki.1001-6821.2017.24.010
- VernacularTitle:塞来昔布对颅脑损伤后生成素表达及神经元凋亡的影响
- Author:
Jin-Ling ZHANG
1
;
Tao ZHANG
;
Jian-Fei GUO
;
Li ZHENG
;
Qun-Fu YANG
;
Fu-Sheng CUI
;
Yu-Xin ZHANG
Author Information
1. 邢台医学高等专科学校药学系,河北邢台054031
- Keywords:
celecoxib;
Survivin;
Caspase-3;
traumatic brain injury;
neuron apoptosis
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(24):2592-2594,2602
- CountryChina
- Language:Chinese
-
Abstract:
Objectve To investigate the effect of celecoxib on the expression of Survivin and neuronal apoptosis after traumatic brain injury in rats,and to explore its brain protection mechanism.Methods The 64 SD rats were randomly divided into four groups:normal group,sham operation group,model group and experimental group.The rats in experimental group and model group were established with closed craniocerebral injury model by free falling method.In the experimental group,intraperitoneal injection of celecoxib (250 mg · kg-1) was performed immediately after the craniocerebral injury model was established.The rats in sham operation group and the normal group were given the same amount of 0.9％ NaC1 intraperitoneally.The expressions of Survivin and Caspase-3 were detected by immunohistochemistry.The neuronal apoptosis was detected by TdT-mediated dUTP-biotin nick end labeling(TUNEL) method.Results The expression of Survivin protein in the normal group,sham-operated group,model group,and experimental group were 3.19 ± 1.43,5.34 ± 1.26,14.17 ±0.54,27.76 ±0.70.The expression of Casepse-3 protein in above four groups were 1.19 ±0.51,3.19 ±0.71,11.17 ±3.74,6.30 ±2.80.The expression of Survivin in the model group was significantly higher than in normal group and sham-operated group with significantly (all P ＜ 0.05).Compared with the model group,the expression of Survivin and Caspase-3 in the experimental group increased with significantly (all P ＜0.05).The number of apoptotic cells in 6,12,24,72 h in the sham-operated group,model group,and experimental group were 2.84 ± 0.56,12.17 ± 1.74,5.36 ± 0.80;3.19 ± 0.71,33.17 ± 3.74,26.30 ± 2.80;5.34 ± 0.83,72.57 ± 6.74,58.48 ± 4.50;2.44 ± 0.83,61.28 ± 5.36,42.56 ± 4.63.The number of apoptotic cells in the model group was significantly higher than in sham-operated group with significantly (all P ＜ 0.05).Compared with the model group,the number of apoptotic cells in the experimental group decreased with significantly (all P ＜ 0.05).Conclusion Celecoxib may inhibit Caspase-3 activation-mediated apoptosis by increasing the expression of anti-apoptotic Survivin protein.In order to reduce the neuronal apoptosis after traumatic brain injury,and play a protective role in the brain.
