Study on the time of cell apoptosis in alcoholic liver injury in mice
10.13699/j.cnki.1001-6821.2017.21.018
- VernacularTitle:酒精性肝损伤小鼠在损伤过程中细胞凋亡时间点研究
- Author:
San-Qiang LI
1
;
Hua-Jie LU
;
Ping WANG
;
Dong-Mei WANG
;
Shan-Long WANG
;
Yong-Yong ZHANG
;
Ying SONG
Author Information
1. 河南科技大学医学院肝损伤与修复分子医学重点实验室
- Keywords:
alcoholic liver injury;
Hoechst33258 staining;
Hematoxylin and eosin staining;
cell apoptosis
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(21):2154-2157
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the changes of hepatocyte apoptosis during alcoholic liver injury in mice.Methods Ninety healthy male KM mice were randomly separated into control group (n =10),model group (n =80).The model group were given 56 ° ethanol (7.5 μL · g-1 · d-1)by gavage for eight weeks,ten of which were dislocated executed at 1,2,3,4,5,6,7,8 weeks,respectively.After the intragastric administration,to take the liver tissue.The mice in the control group were sacrificed after the same amount of distilled water,and the liver was tested and analyzed.Changes of cell apoptosis of alcoholic-incuded liver injury were detected by Hematoxylin and eosin (HE) and Hoechst33258 staining.To identify and count of apoptotic cells and normal cells under a fluorescence microscope,which can be used to calculate the rate of apoptosis.Results Acute hepatic injury were observed at third week,manifested as increased inflammatory cell infiltration.The eighth week showed chronic liver damage,characterized by degeneration of liver tissue water and fat vacuoles.The apoptotic rates of the model group were (9.28 ±0.41)％,(13.20 ±0.81)％ and (20.43 ±0.97)％,at 1-3 weeks,respectively.Compared with the control group (1.38 ±0.18)％,hepatocyte apoptosis was significantly increased at three weeks (all P ＜0.05).Liver cell apoptosis rate increased gradually,with the extension of the time.Apoptosis rate reached (51.99 ± 1.70) ％ at eight weeks,which was significantly different from that of the control group (P ＜ 0.01).Conclusion The degree of liver injury induced by alcohol is closely related to the development of liver cell apoptosis.
