Effect of scorpion venom peptide on the invasion and metastasis of gastric cancer cell line SGC-7901
10.13699/j.cnki.1001-6821.2016.13.016
- VernacularTitle:蝎毒多肽对胃癌 SGC－7901细胞侵袭转移的作用
- Author:
Zhen-Lü LIN
1
;
Guang-Wei ZHENG
;
Lin ZHANG
;
Jian-Tao ZHENG
;
Hui CHEN
Author Information
1. 福建医科大学 附属第一医院 急诊外科
- Keywords:
gastric cancer;
invasion;
metastasis;
scorpion venom peptide
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(13):1206-1209
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of scorpion venom peptide on the invasion and metastasis of gastric cancer cell lines SGC -7901. Methods One blank group and 5 concentrations of ( scorpion venom polypeptide, 5,10,20,40,80 μg? mL-1 ) test groups were set up.The 3-(4,5-dimethyl-2-thiazolyl) -2,5-diphenyl -2-H-tetrazoli-um bromide ( MTT assay ) was used to detect the effects of scorpion venom peptide on the activity and proliferation of cells , while its effects on cell invasion and metastasis were observed by scratch test and Transwell test . The expressions of matrix metalloproteinase -2 (MMP-2) and matrix metalloproteinase -9 ( MMP -9 ) were observed by Western blot . Results Compared with the blank group , the scorpion venom peptide could significantly inhibit the proliferation of gastric cancer SGC -7901 cells and show the time-concentration correlation(P<0.05).Two con-centrations of scorpion venom peptide (10, 20 μg? mL-1 ) could inhibit the invasive ability of the cell , with invasion of the cell number (35.8 ± 5.53 ) , ( 19.3 ±2.13 ) , respectively , compared with the blank group (48.3 ±7.34), the difference among the groups was statistically signifi-cant ( P <0.05 ) . It means the two test groups could inhibit the migration ability of gastric cancer cell lines SGC -7901.Compared with blank group , the migration inhibitory rates of the two concentration ( 10, 20 μg? mL-1 ) of test groups were significantly lower with ( 1.47 ±1.23 )%, (28.5 ±3.35 )%, respectively.Compared with the blank group , those in two test groups could inhibit the expressions of MMP-9 and MMP-2 cells at the time of 12 hours after administration by scorpion venom peptide on gastric cancer SGC-7901 cells ( P<0.05 ) .Conclusion The scorpion venom peptide can inhibit the invasion and metastasis of gastric cancer SGC-7901 cells by inhibiting the expressions of MMP -2 and MMP-9.
