Pharmacokinetics study of levosulpiride in human plasma by HPLC-MS/MS
10.13699/j.cnki.1001-6821.2015.24.019
- VernacularTitle:高效液相色谱－质谱联用法测定人血浆中左舒必利的浓度
- Author:
Wen-Yan HUA
1
;
Quan-Ying ZHANG
;
Shun-Lin ZONG
;
Ming HUANG
;
Meng WANG
Author Information
1. 苏州大学 附属第二医院 临床试验机构
- Keywords:
levosulpiride;
HPLC-MS/MS;
pharmacokinetics
- From:
The Chinese Journal of Clinical Pharmacology
2015;(24):2430-2432
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC-MS/MS method for de-termination of levosulpiride in human plasma.Methods After protein precipitation, the plasma was separated on Xterra ? RP18 (4.6 mm × 150 mm, 5 μm ) column, with a mobile phase of acetonitrile -10 mmoL? L-1 ammonium acetate containing 0.2% formic acid ( 10∶90 ) . The flow rate was 1.0 mL? min -1 and the column temperature was 30℃.Quantification was performed in the positive ion multiple reaction mo-nitoring( MRM) mode.Results Determination of levosulpiride had good linearity in the concentration range of 1.00 -600.00 μg? L-1 .The standard curve was y=2.93 ×10 -3 x+1.75 ×10 -2 ( n=3,r=0.999 3). The lower limit of quantization was 1.00 μg? L-1 , extraction recovery rate was 92.12%-98.53%, and intra -batch and inter-batch RSD were both less than 4.61%. Conclusion The method is specific, simple, sensitive, rapid, accurate and suitable for determination of levosulpiride in human plasma.
